Synergistic bacterial compositions and methods of production and use thereof
Provided are therapeutic compositions containing Ecobiotic™ populations for prevention, treatment and reduction of symptoms associated with a dysbiosis of a mammalian subject such as a human.
1. A composition comprising a first species of isolated bacterium and a second species of isolated bacterium and a capsule, which encapsulates the first species, the second species, or both the first and second species of isolated bacterium, wherein the first species is Dorea longicatena and the second species is Clostridium orbiscindens , wherein the Clostridium orbiscindens comprises a 16S rDNA sequence that is at least 97% identical to SEQ ID NO: 548, and wherein the first species and the second species are capable of inhibiting Clostridium difficile ( C. difficile ) growth as measured by a CivSim assay.
2. The composition of claim 1 , which further comprises an enteric coating.
3. The composition of claim 1 , wherein the first species and the second species are capable of inhibiting C. difficile growth in the CivSim assay with a confidence interval of >99% (++++).
4. The composition of claim 1 , wherein the first species, the second species, or both are in the form of spores.
5. The composition of claim 1 , which is formulated for populating the gastrointestinal tract of the subject.
6. The composition of claim 1 , wherein the first species, the second species, or both are lyophilized.
7. A kit comprising in one or more containers the composition of claim 1 .
8. A pharmaceutical formulation comprising the composition of claim 1 .
9. The pharmaceutical formulation of claim 8 , further comprising an additional agent.
10. The pharmaceutical formulation of claim 9 , wherein the additional agent is selected from an anti-bacterial agent, anti-fungal agent, anti-viral agent, anti-parasitic agent, or combinations thereof.
11. The method of claim 1 , wherein the Dorea longicatena comprises a 16S rDNA sequence that is at least 97% identical to SEQ ID NO: 705.
12. The method of claim 1 , wherein the Dorea longicatena comprises a 16S rDNA sequence that is at least 98% identical to SEQ ID NO: 705.
13. The method of claim 1 , wherein the Dorea longicatena comprises a 16S rDNA sequence that is at least 99% identical to SEQ ID NO: 705.
14. The method of claim 1 , wherein the Dorea longicatena comprises the 16S rDNA sequence set forth in SEQ ID NO: 705.
15. The composition of claim 1 , wherein the Clostridium orbiscindens comprises a 16S rDNA sequence that is at least 98% identical to SEQ ID NO: 548.
16. The composition of claim 1 , wherein the Clostridium orbiscindens comprises a 16S rDNA sequence that is at least 99% identical to SEQ ID NO: 548.
17. The composition of claim 1 , wherein the Clostridium orbiscindens comprises the 16S rDNA sequence set forth in SEQ ID NO: 548.
18. The pharmaceutical formulation of claim 8 , which further comprises a pharmaceutically acceptable excipient.
19. The pharmaceutical formulation of claim 8 , which is formulated for oral administration.
20. The composition of claim 1 , which further comprises glycerol.