Buffer management system with manifold for bioprocessing system
A buffer management system includes at least one workstation configured to support a supply of concentrated buffer solution and an inline dilution skid having a manifold. The manifold is in fluid communication with the supply of concentrated buffer solution and is adapted to be placed in fluid communication with a source of water for injection (WFI). The manifold includes a buffer inlet line in selective fluid communication with the supply of concentrated buffer solution, a WFI inlet line in selective fluid communication with the source of WFI, and a discharge line in fluid communication with both the buffer inlet line and the WFI inlet line. The inline dilution skid is configured to variably mix the supply of concentrated buffer solution and the source of WFI to obtain a range of diluted buffer solutions.
1 . A manifold for a buffer management system, the manifold comprising:
a mixing junction;
a buffer inlet line, the buffer inlet line in fluid communication with the mixing junction;
a water for injection (WFI) inlet line, the WFI inlet line in fluid communication with the mixing junction;
a discharge line, the discharge line in fluid communication with both the buffer inlet line and the WFI inlet line via the mixing junction;
a first pump component, the first pump component disposed in the buffer inlet line, the first pump component configured to be operably arranged with a first pump motor to selectively generate a fluid displacement within the buffer inlet line;
a second pump component, the second pump component disposed in the WFI inlet line, the second pump component configured to be operably arranged with a second pump motor to selectively generate a fluid displacement within the WFI inlet line;
a buffer characteristic sensor, the buffer characteristic sensor disposed in the discharge line, the buffer characteristic sensor configured to detect a value of a buffer characteristic of a fluid in the discharge line and to generate a buffer characteristic signal indicative of the sensed value for the buffer characteristic;
wherein the buffer inlet line includes a plurality of buffer inlet ports in fluid communication therewith, the WFI inlet line includes a water port in fluid communication therewith, and the discharge line includes a plurality of buffer discharge ports in fluid communication therewith, the plurality of buffer discharge ports corresponding to the plurality of buffer inlet ports.
2 . The manifold according to claim 1 , wherein the buffer inlet line, the WFI inlet line, and the discharge line comprise tubing adapted to be selectively occluded by a pinch valve externally mounted thereto.
3 . The manifold according to claim 1 , further comprising:
a drain junction, the drain junction in fluid communication with the discharge line;
a waste line, the waste line being in fluid communication with the discharge line via the drain junction.
4 . The manifold according to claim 3 , wherein the buffer characteristic sensor comprises a first buffer characteristic sensor, the manifold further comprising:
a second buffer characteristic sensor, the second buffer characteristic sensor disposed in the waste line, the second buffer characteristic sensor configured to detect a value of the buffer characteristic of a fluid in the waste line and to generate a second buffer characteristic signal indicative of the sensed value for the buffer characteristic in the waste line.
5 . The manifold according to claim 4 , wherein the first buffer characteristic sensor and the second buffer characteristic sensor both comprise a conductivity sensor.
6 . The manifold according to claim 4 , wherein the drain junction comprises a first drain junction, the manifold further comprising:
a second drain junction, the second drain junction in fluid communication with both the WFI line and the waste line such that the WFI line is in fluid communication with the waste line via the second drain junction.
7 . The manifold according to claim 6 , wherein the buffer inlet line is in fluid communication with the waste line.
8 . The manifold according to claim 6 , further comprising:
a third drain junction, the third drain junction in fluid communication with, and interposed between, the buffer inlet line and the WFI inlet line;
wherein the buffer inlet line is in fluid communication with the waste line via the third drain junction, the WFI line, and the second drain junction.