Variant antibody that binds CD38
The present disclosure provides anti-CD38 IgG class antibody having improved ability to be manufactured at higher yields compared to the parent antibody having the original wild type sequence. The present disclosure provides a mutated antibody light chain that reduces cleavage heterogeneity for improved production of a homogeneous population of antibody light chains.
1 . An antigen binding protein that binds CD38, comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises the sequence of SEQ ID NO: 1 and the light chain variable region comprises the sequence of SEQ ID NO: 3.
2 . The antigen binding protein of claim 1 , which is a fully human single chain antibody, comprising a heavy chain comprising a heavy chain variable region having the amino acid sequence of SEQ ID NO:1, and a light chain comprising a light chain variable region having the amino acid sequence of SEQ ID NO:3.
3 . A pharmaceutical composition comprising a pharmaceutically-acceptable excipient and the antigen binding protein of claim 1 .
4 . A fully human antibody of an IgG class or antibody Fab fragment thereof, comprising a heavy chain comprising a heavy chain variable region having the amino acid sequence of SEQ ID NO:1, and a light chain comprising a light chain variable region having the amino acid sequence of SEQ ID NO:3.
5 . The fully human antibody or antibody Fab fragment thereof of claim 4 , which is a fully human antibody.
6 . The fully human antibody or antibody Fab fragment thereof of claim 4 , which is a Fab fragment.
7 . A nucleic acid encoding the fully human antibody of claim 4 .
8 . A nucleic acid encoding an antibody light chain comprising a light chain variable region having the amino acid sequence of SEQ ID NO:3.
9 . The nucleic acid of claim 8 , further comprising a nucleic acid encoding an antibody heavy chain which comprises the amino acid sequence of SEQ ID NO:1.
10 . An expression vector comprising a promoter operably linked to the nucleic acid of claim 8 .
11 . The expression vector of claim 10 further comprising a nucleic acid encoding an antibody heavy chain which comprises the amino acid sequence of SEQ ID NO: 1.
12 . An isolated host cell comprising the expression vector of claim 11 .
13 . A method for preparing a fully human antibody light chain, comprising: culturing a population of the host cell of claim 12 under conditions suitable for expressing the antibody light chain.
14 . The method of claim 13 , further comprising recovering from the host cells the expressed antibody light and heavy chains.
15 . The method of claim 14 , wherein the percentage of the recovered expressed antibody light chains having the correct length, the correct N-terminal amino acid, and having 100% sequence identity to the light chain variable domain of SEQ ID NO: 3 is 90-100%.
16 . An isolated host cell comprising the expression vector of claim 10 .
17 . A method for preparing a fully human antibody light chain, comprising: culturing a population of the host cell of claim 16 under conditions suitable for expressing the antibody light chain.
18 . The method of claim 17 , further comprising recovering from the host cells the expressed antibody light chain.
19 . The method of claim 18 , wherein the percentage of the recovered expressed antibody light chains having the correct length, the correct N-terminal amino acid, and having 100% sequence identity to the light chain variable domain of SEQ ID NO: 3 is 90-100% and/or wherein the homogeneity of the recovered expressed antibody light chains is 90-100%.