Compositions comprising bacterial strains
The invention provides compositions comprising bacterial strains for treating and preventing lung injury or lung disease.
1. A method of treating lung injury or lung disease in a subject in need thereof, comprising administering to the subject a pharmaceutical composition comprising a lyophilized bacterial strain of the genus Blautia , wherein the lyophilized bacterial strain comprises a polynucleotide sequence of a 16S rRNA gene that has at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO:1, and a pharmaceutically acceptable excipient, diluent, or carrier,
wherein the lung injury or the lung disease is non-asthma lung injury or non-asthma lung disease.
2. The method of claim 1 , wherein the lung injury or the lung disease is mediated by inflammation in a lung tissue of the subject.
3. The method of claim 2 , wherein the pharmaceutical composition reduces inflammation in the lung tissue.
4. The method of claim 2 , wherein the lung injury or the lung disease comprises bronchiectasis, bronchiolitis, bronchitis, chronic cough, cystic fibrosis, emphysema, human metapneumovirus (h1VIPV) infection, influenza, pertussis, pleural cavity diseases, pneumonia, respiratory syncytial virus (RSV), sarcoidosis, severe acute respiratory syndrome (SARS), tuberculosis, or upper respiratory tract infections.
5. The method of claim 1 , wherein the lung injury or the lung disease comprises acute respiratory distress syndrome (ARDS) or chronic obstructive pulmonary disease (COPD).
6. The method of claim 1 , wherein the administering the pharmaceutical composition reduces one or more of neutrophil inflammation, haemorrhage, oedema, necrosis, and atelectasis in the lung tissue.
7. The method of claim 6 , wherein the administering the pharmaceutical composition reduces all of neutrophil inflammation, haemorrhage, oedema, necrosis, and atelectasis in the lung tissue.
8. The method of claim 1 , wherein the bacterial strain has a 16s rRNA gene sequence that has at least 98% sequence identity to the polynucleotide sequence of SEQ ID NO:1, as determined by a Smith-Waterman homology search algorithm using an affine gap search with a gap open penalty of 12 and a gap extension penalty of 2.
9. The method of claim 1 , wherein the bacterial strain has a 16s rRNA gene sequence comprising the polynucleotide sequence of SEQ ID NO:1.
10. The method of claim 1 , wherein the pharmaceutical composition comprises a single strain of the genus Blautia.
11. The method of claim 1 , wherein the pharmaceutical composition comprises the bacterial strain of the genus Blautia as a part of a microbial consortium.
12. The method of claim 1 , wherein the bacterial strain is of the species Blautia hydrogenotrophica.
13. The method of claim 12 , wherein the bacterial strain is the strain deposited as accession number DSM 14294 at the Deutsche Sammlung von Mikroorganismen (German Microorganism Collection), a biotype thereof, or a derivative thereof.
14. The method of claim 1 , wherein the pharmaceutical composition is formulated for oral delivery.
15. The method of claim 1 , wherein the bacterial strain is viable.
16. The method of claim 1 , wherein the bacterial strain is capable of colonizing the intestine.
17. The method of claim 1 , wherein the 95% sequence identity is determined by a Smith-Waterman homology search algorithm using an affine gap search with a gap open penalty of 12 and a gap extension penalty of 2.
18. A method of reducing damage in a lung tissue caused by lung injury or lung disease in a subject in need thereof, comprising administering to the subject a pharmaceutical composition comprising a lyophilized bacterial strain of the genus Blautia , wherein the lyophilized bacterial strain comprises a polynucleotide sequence of a 16S rRNA gene that has at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO:1, and a pharmaceutically acceptable excipient, diluent, or carrier,
wherein the lung injury or the lung disease is non-asthma lung injury or non-asthma lung disease.
19. The method of claim 18 , wherein the lung injury or the lung disease comprises acute respiratory distress syndrome (ARDS) or chronic obstructive pulmonary disease (COPD).
20. The method of claim 4 , wherein the influenza is pandemic flu and the pleural cavity disease is pleurisy.