Methods and compositions for microbial engraftment
The disclosure provides compositions and methods for therapeutic administration of the compositions to treat comorbidities associated with gut dysbiosis. The disclosure also provides compositions and methods for increasing engraftment of administered microbes. The disclosure also provides methods for stool sample collection.
1. A method of increasing engraftment of Eubacterium hallii in the gut of a mammalian subject, the method comprising:
administering to the gut of the mammalian subject Eubacterium hallii and Akkermansia muciniphila ; and
measuring increased engraftment of Eubacterium hallii in the gut of the subject.
2. The method according to claim 1 , wherein the engraftment is increased by at least about 5%.
3. The method according to claim 1 , wherein the increased engraftment is measured by measuring an increased relative abundance of the at least one butyrate producing microbe Eubacterium hallii in the subject for more than 4 weeks after administration has ceased.
4. The method according to claim 1 , wherein the Eubacterium hallii only engrafts in a presence of Akkermansia muciniphila.
5. The method according to claim 1 , wherein the Eubacterium hallii and Akkermansia muciniphila are formulated for oral delivery.
6. The method according to claim 1 , further comprising administering a prebiotic.
7. The method according to claim 6 , wherein the prebiotic is inulin.