Immunogenic compositions to treat and prevent microbial infections
The invention relates to composite antigens comprising a peptide with contiguous amino acid sequence derived from a plurality of antigenic epitopes of one or more pathogens that induces an immune response in a mammal that is protective against infection by the one or more pathogens. In addition. The invention also relates to antibodies to composite antigens of the invention and to methods of administering vaccines comprising antigens or vaccines of antibodies for treating and/or preventing an infection.
1. A peptide containing a plurality of different microbial epitopes all as a single peptide sequence, wherein the plurality comprises a corona virus epitope and an influenza virus epitope.
2. The peptide of claim 1 , wherein the influenza virus epitope is an epitope of an HA protein, an NA protein, an M1 protein, an M2 protein, or an M2e protein of an influenza virus, and/or a fragment, derivative, or modification thereof.
3. The peptide of claim 1 , further comprising a T cell stimulating epitope.
4. The peptide of claim 3 , wherein the T cell stimulating epitope is at the N-terminus or the C-terminus of the peptide.
5. The peptide of claim 3 , wherein the T cell stimulating epitope comprises the sequence of tetanus toxin, tetanus toxin heavy chain proteins, diphtheria toxoid, CRM, recombinant CRM, tetanus toxoid, Pseudomonas exoprotein A, Pseudomonas aeruginosa toxoid, Bordetella pertussis toxoid, Clostridium perfringens toxoid, Escherichia coli heat-labile toxin B subunit, Neisseria meningitidis outer membrane complex, Hemophilus influenzae protein D, Flagellin Fli C, Horseshoe crab Haemocyanin, and/or a fragment, derivative, or modification thereof.
6. The peptide of claim 1 , which comprises an immunogenic composition reactive against both an influenza virus infection and a corona virus infection.
7. The immunogenic composition of claim 6 , comprising an adjuvant.
8. The immunogenic composition of claim 6 , wherein the adjuvant comprises Freund's, a liposome, saponin, lipid A, squalene, and derivatives and combinations thereof.
9. The immunogenic composition of claim 6 , which is a vaccine that treats or prevents both an influenza virus infection and a corona virus infection.
10. The peptide of claim 1 , which comprises multiple epitopes of influenza virus.
11. The peptide of claim 1 , which comprises multiple epitopes of corona virus.
12. The peptide of claim 1 , wherein the plurality comprises an epitope of a corona virus polymerase protein and an epitope of an influenza virus matrix protein.
13. The peptide of claim 1 , wherein the corona virus epitopes is an epitope of a spike protein (S), an envelope protein (E), a membrane protein (M), a nucleocapsid protein (N), or a polymerase protein (P) of a of corona virus and/or a fragment, derivative, or modification thereof.
14. The peptide of claim 1 , wherein the sequence of the influenza virus epitope is selected from the group consisting of any sequence of SEQ ID NOs. 14-29, and combinations thereof.
15. The peptide of claim 1 , wherein the sequence of the corona virus epitope is selected from the group consisting of any sequence of SEQ ID NOs. 9-13, 34-36, and 38-45 and combinations thereof.
16. The peptide of claim 1 , wherein the sequence of the peptide is selected from the group consisting of any sequence of SEQ ID NOs. 37 and 46-48, and combinations thereof.
17. The peptide of claim 1 , wherein the influenza virus epitope is a composite epitope.
18. A method to treat or prevent a microbial infection by administering the immunogenic composition of claim 6 to a mammal suspected of being or determined to be infected with a pathogen.
19. The method of claim 6 , wherein the immunogenic composition produces a neutralizing response by the mammal against the pathogen.
20. A peptide containing as adjacent epitopes a corona virus epitope, an influenza virus epitope, and a T cell stimulating epitope as a single sequence.
21. The peptide of claim 20 , wherein the corona virus epitope is a composite epitope.
22. The peptide of claim 20 , wherein the corona virus epitope is obtained or derived from a spike protein (S), an envelope protein (E), a membrane protein (M), a polymerase protein (P), a nucleocapsid protein (N) of coronavirus, and/or a fragment, derivative, or modification thereof.
23. The peptide of claim 20 , wherein the influenza virus epitope is obtained or derived from an HA protein, an NA protein, an M1 protein, an M2 protein, an M2e protein of the influenza virus, and/or a fragment, derivative, or modification thereof.
24. The peptide of claim 20 , wherein the T cell stimulating epitope is obtained or derived from tetanus toxin, tetanus toxin heavy chain proteins, diphtheria toxoid, CRM, recombinant CRM, tetanus toxoid, Pseudomonas exoprotein A, Pseudomonas aeruginosa toxoid, Bordetella pertussis toxoid, Clostridium perfringens toxoid, Escherichia coli heat-labile toxin B subunit, Neisseria meningitidis outer membrane complex, Hemophilus influenzae protein D, Flagellin Fli C, Horseshoe crab Haemocyanin, and/or a fragment, derivative, or modification thereof.
25. The peptide of claim 20 , wherein the T cell stimulating epitope is at the N-terminus or the C-terminus of the peptide.
26. The peptide of claim 20 , which comprises multiple influenza virus epitopes, multiple corona virus epitopes, and/or multiple T cell stimulating epitope.
27. The peptide of claim 20 , which comprises an immunogenic composition.
28. The immunogenic composition of claim 27 , comprising an adjuvant.
29. The immunogenic composition of claim 28 , wherein the adjuvant comprises Freund's, a liposome, saponin, lipid A, squalene, and derivatives and combinations thereof.
30. The immunogenic composition of claim 27 , which is a vaccine that treats or prevents a corona virus infection.
31. The peptide of claim 20 , which comprises multiple epitopes of influenza virus.
32. The peptide of claim 20 , which comprises multiple epitopes of corona virus.
33. The peptide of claim 20 , wherein the influenza virus epitope is a composite epitope.
34. The peptide of claim 20 , wherein the corona virus epitope is a composite epitope.
35. The peptide of claim 1 , wherein the corona virus epitope comprises an epitope of a corona virus spike protein.
36. The peptide of claim 1 , wherein the corona virus epitope comprising an epitope of a corona virus polymerase protein.
37. The peptide of claim 1 , wherein the influenza virus epitope comprising an epitope of an influenza virus HA protein.
38. The peptide of claim 1 , wherein the influenza virus epitope comprising an epitope of an influenza virus NA protein.
39. The peptide of claim 1 , wherein the influenza virus epitope comprising an epitope of an influenza virus matrix protein.
40. The peptide of claim 1 , which comprises epitopes of matrix, HA and/or NA proteins of influenza virus and epitopes of spike protein and/or polymerase protein of corona virus.
41. The peptide of claim 1 , which contains the sequence of SEQ ID NO. 19.
42. The peptide of claim 1 , which contains the sequence of SEQ ID NO. 21.
43. The peptide of claim 1 , wherein the corona virus epitope comprises an epitope of corona virus spike protein and the influenza virus epitope comprises epitopes of influenza virus NA and HA proteins.
44. The peptide of claim 1 , wherein the corona virus epitope comprises an epitope of corona virus spike protein and the influenza virus epitope comprises an epitope of an influenza virus matrix protein.
45. The peptide of claim 1 , wherein the corona virus epitope comprises an epitope of corona virus polymerase protein and the influenza virus epitope comprises epitopes of influenza virus NA and HA proteins.
46. The peptide of claim 1 , wherein the corona virus epitope comprises an epitope of corona virus polymerase protein and the influenza virus epitope comprises an epitope of influenza virus matrix protein.
47. The peptide of claim 1 , wherein the corona virus epitope comprises epitopes of corona virus polymerase and spike proteins and the influenza virus epitope comprises epitopes of influenza virus NA and matrix proteins.
48. The peptide of claim 1 , wherein the corona virus epitope comprises epitopes of corona virus polymerase and spike proteins and the influenza virus epitope comprises epitopes of influenza virus HA and matrix proteins.
49. The peptide of claim 1 , wherein the corona virus epitope comprises epitopes of corona virus polymerase and spike proteins and the influenza virus epitope comprises epitopes of influenza virus HA, NA and matrix proteins.