Insecticidal proteins
Compositions and methods for controlling plant pests are disclosed. In particular, novel engineered hybrid insecticidal proteins having toxicity to at least European corn borer are provided. By fusing unique combinations of complete or partial variable regions and conserved blocks of at least two different Bacillus thuringiensis (Bt) Cry proteins or a modified Cry proteins a hybrid insecticidal protein having activity against at least European corn borer is designed. Nucleic acid molecules encoding the novel hybrid proteins are also provided. Methods of making the hybrid proteins and methods of using the hybrid proteins and nucleic acids encoding the hybrid proteins of the invention, for example in transgenic plants to confer protection from insect damage are also disclosed.
1. An engineered hybrid insecticidal protein comprising the amino acid sequence of SEQ ID NO:4.
2. A nucleic acid comprising a coding sequence that encodes the engineered hybrid insecticidal protein of claim 1 .
3. The nucleic acid of claim 2 , wherein the coding sequence comprises SEQ ID NO:3.
4. An expression cassette comprising a heterologous promoter sequence operably linked to the nucleic acid molecule of claim 2 .
5. A vector comprising the expression cassette of claim 4 .
6. A transgenic host cell comprising the engineered hybrid insecticidal protein of claim 1 .
7. A transgenic host cell comprising the expression cassette of claim 4 .
8. The transgenic host cell of claim 7 , wherein the transgenic host cell is a plant cell.
9. The transgenic host cell of claim 8 , wherein the plant cell is a monocot cell.
10. The transgenic host cell of claim 9 , wherein the plant cell is a maize cell.
11. A transgenic plant comprising the engineered hybrid insecticidal protein of claim 1 .
12. The transgenic plant of claim 11 , wherein the plant is a monocot.
13. The transgenic plant of claim 12 , wherein the plant is a maize plant.
14. A seed of the transgenic plant of claim 11 , wherein the seed comprises the engineered hybrid insecticidal protein.
15. A seed of the transgenic plant of claim 12 , wherein the seed comprises the engineered hybrid insecticidal protein.
16. A seed of the transgenic plant of claim 13 , wherein the seed comprises the engineered hybrid insecticidal protein.
17. An insecticidal composition comprising the engineered hybrid insecticidal protein of claim 1 .
18. A method of producing an insect-resistant transgenic plant, comprising:
(a) introducing the expression cassette according to claim 4 into a plant genome thus producing a transgenic plant; and
(b) expressing the engineered hybrid insecticidal protein in an effective amount to control insects.
19. A method of controlling a coleopteran pest comprising delivering to the pest the engineered hybrid insecticidal protein of claim 1 .
20. The method of claim 19 , wherein the engineered hybrid insecticidal protein is delivered by feeding.