IP Library › Granted Patent US 12,241,100
Granted Patent B2
US 12,241,100 · App. 17/166,934 · Granted Mar 4, 2025

Engineered leucine decarboxylases

Inventors: Joyce Liu (Fremont, CA); Leann Quertinmont Teadt (Redwood City, CA); Nikki Dellas (San Carlos, CA); Stephan Jenne (Foster City, CA); Faye Loan Du (San Jose, CA); Kristen Jean Vallieu (Union City, CA); Kerryn McCluskie (Pacifica, CA)
Assignee: SYNTIS BIO, INC.
C12N9/88A61K38/00C12Y401/01014
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Quick Facts
Patent No.
US 12,241,100
App. No.
17/166,934
Granted
Mar 4, 2025
Kind
B2
Abstract

The present invention provides engineered leucine decarboxylase (LDC) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered leucine decarboxylase (LDC) polypeptides. In some embodiments, the engineered LDC polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis, and/or increased tolerance to low pH environments. In some embodiments, the engineered LDC polypeptides are optimized to provide improved storage stability. The present invention also provides methods for the use of the compositions comprising the engineered LDC polypeptides for therapeutic and industrial purposes.

Claims (6)

1. An engineered leucine decarboxylase polypeptide comprising an amino acid sequence having at least 90%, 91%, 92,%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to the entire sequence set forth in SEQ ID NO: 12, wherein the amino acid positions of said amino acid sequence are numbered with reference to the amino acid sequence of SEQ ID NO: 12, and wherein said amino acid sequence comprises a substitution at position 364, selected from L364K and L364R.

2. The engineered leucine decarboxylase polypeptide of claim 1 , wherein said engineered leucine decarboxylase polypeptide exhibits more activity on leucine than the leucine decarboxylase polypeptide set forth in SEQ ID NO: 12.

3. The engineered leucine decarboxylase polypeptide of claim 1 , wherein said engineered leucine decarboxylase polypeptide is more resistant to proteolysis than the leucine decarboxylase polypeptide set forth in SEQ ID NO: 12.

4. The engineered leucine decarboxylase polypeptide of claim 1 , wherein said engineered leucine decarboxylase polypeptide is more thermostable than the leucine decarboxylase polypeptide set forth in SEQ ID NO: 12.

5. The engineered leucine decarboxylase polypeptide of claim 1 , wherein said engineered leucine decarboxylase polypeptide has been isolated from a recombinant expression host.

6. A composition comprising at least one engineered leucine decarboxylase polypeptide provided in claim 1 .

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 7, 2024
From: CODEXIS, INC.
To: SYNTIS BIO, INC.
Reel/Frame 067340/0445 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 20, 2021
From: LIU, JOYCE; TEADT, LEANN QUERTINMONT; DELLAS, NIKKI; JENNE, STEPHAN; DU, FAYE LOAN; VALLIEU, KRISTEN JEAN; MCCLUSKIE, KERRYN
To: CODEXIS, INC.
Reel/Frame 055973/0405 →
Continuity (2)
Provisional Application 62970039 · Feb 4, 2020
Related Publication 20210238575A1 · Aug 5, 2021
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