Oil-based adjuvants
The instant invention provides various formulations comprising combinations of immunostimulating oligonucleotides, polycationic carriers, sterols, saponins, quaternary amines, TLR-3 agonists, glycolipids, and MPL-A or analogs thereof in oil emulsions, use thereof in preparations of immunogenic compositions and vaccines, and use thereof in the treatment of animals.
1. A vaccine composition comprising a Rhipicephalus microplus antigen and an adjuvant, said adjuvant being selected from the group consisting of:
a) an aqueous adjuvant comprising an immunostimulatory oligonucleotide, a saponin, a sterol, a quaternary amine, a polyacrylic polymer, and a glycolipid; and
b) an oil-based adjuvant, comprising an oily phase present in the amount of at least 45% v/v of the vaccine composition and comprising an immunostimulatory oligonucleotide and a polycationic carrier, wherein said vaccine composition is a water-in-oil emulsion.
2. The vaccine composition of claim 1 , wherein the saponin is Quil A, the sterol is cholesterol, the quaternary amine is DDA, the glycolipid is N-(2-Deoxy-2-L-leucylamino-b-D-glucopyranosyl)-N-octadecyldodecanoylamide or a salt thereof, and the immunostimulatory oligonucleotide is a CpG.
3. The vaccine composition of claim 1 , wherein the polycationic carrier is dextran DEAE and the immunostimulatory oligonucleotide is a CpG.
4. A method treatment or prevention of an infection caused by Rhipicephalus microplus , said method comprising administering to a subject in need thereof a vaccine composition according to claim 1 .
5. The vaccine composition of claim 1 wherein the oily phase comprises at least 48% v/v of said vaccine composition.
6. The vaccine composition of claim 1 wherein the oily phase comprises at least 50% v/v of said vaccine composition.
7. The vaccine composition of claim 6 wherein the oily phase comprises 50-52% of said vaccine composition.