IP Library › Granted Patent US 12,290,557
Granted Patent B2
US 12,290,557 · App. 17/177,724 · Granted May 6, 2025

Vaccine compositions for porcine epidemic diarrhea virus and porcine deltacoronavirus

Inventors: Jacqueline Gayle Marx (Portage, MI); John Morgan Hardham (Kalamazoo, MI); Paul J. Dominowski (Kalamazoo, MI); Vicki Jon Rapp Gabrielson (Kalamazoo, MI); Monica Balasch Sanuy (Barcelona, ES); Marta Cabana Sumsi (Barcelona, ES); Laia Plaja Dilmé (Girona, ES); Alicia Urniza Hostench (Girona, ES); Oscar Romero Galindo (White Plains, NY)
Assignee: Zoetis Services LLC
A61K39/215A61K39/12C12N7/00A61K2039/5252A61K2039/552A61K2039/55505A61K2039/55566A61K2039/575A61K2039/70C12N2770/20022C12N2770/20034C12N2770/20063C12N2770/20071
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Quick Facts
Patent No.
US 12,290,557
App. No.
17/177,724
Granted
May 6, 2025
Kind
B2
Abstract

The present invention is directed to novel immunogenic compositions that protect swine from disease caused by porcine epidemic diarrhea virus (PEDV). The present invention is also directed to novel immunogenic compositions that protect swine from disease caused by porcine deltacoronavirus (PDCoV), alone or as combination vaccine to protect against PEDV. The compositions of the invention provide killed viruses whose effectiveness is enhanced by the selection of preferred adjuvants. Novel culture methods are also employed to increase reproducible yield of cultured viruses. Live vaccines are also provided from the Calaf14 PEDV isolate.

Claims (6)

1. A vaccine composition comprising inactivated porcine epidemic diarrhea virus (PEDV), adjuvanted as an oil-in-water emulsion, wherein the adjuvant components include de-oiled lecithin dissolved in a light mineral oil and aluminum hydroxide, wherein said vaccine, upon administration to a swine, elicits production of neutralizing antibodies in the swine.

2. The vaccine composition of claim 1 wherein the inactivated PEDV is strain USA/Colorado/2013, whose genomic sequence is deposited in GenBank as accession No. KF272920 and whose genomic sequence comprises SEQ ID NO: 7.

3. The vaccine composition of claim 1 wherein the final concentration of 20% de-oiled lecithin dissolved in a light mineral oil is 25% (v/v).

4. The vaccine composition of claim 1 further comprising inactivated porcine deltacoronavirus (PDCoV).

5. The vaccine composition of claim 4 wherein the PDCOV is selected from the group consisting of USA/Michigan/8977/2014, whose sequence is deposited as GenBank accession No. KM012168 and whose genomic sequence comprises SEQ ID NO:12, and strain USA/Indiana/2014/8501010, whose genome sequence comprises SEQ ID NO: 11.

6. The vaccine composition of claim 4 wherein the final concentration of 20% de-oiled lecithin dissolved in a light mineral oil is 25% (v/v).

Continuity (11)
Continuation 16282953 · Feb 22, 2019
Continuation 15324908
Provisional Application 62143412 · Apr 6, 2015
Provisional Application 62121193 · Feb 26, 2015
Provisional Application 62115806 · Feb 13, 2015
Provisional Application 62102712 · Jan 13, 2015
Provisional Application 62093657 · Dec 18, 2014
Provisional Application 62046256 · Sep 5, 2014
Provisional Application 62037403 · Aug 14, 2014
Provisional Application 62023302 · Jul 11, 2014
Related Publication 20240226270A1 · Jul 11, 2024
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