Manipulation of sample droplets with an electrode system
A system and method for sample droplet processing, the system including a substrate, an electrode array network coupled to the substrate and configured to provide a pattern of controlled electric fields for manipulation of the set of sample droplets; a first layer in communication with the electrode array network, the first layer separating the electrode array network from fluid of the set of sample droplets; and a second layer opposing the first layer and displaced from the first layer to define a region wherein droplets of the set of sample droplets can reside. In some variations, the system can additionally include an electronics subsystem coupled to at least one of the substrate and the electrode array network, and a control module in communication with the electronics subsystem, wherein the control module generates and manipulates the pattern of controlled electric fields.
1 . A method for processing a set of sample droplets, the method including:
transmitting a sample droplet of a set of sample droplets from a sample reservoir into a region between a first layer and a second layer of a magnetic separation unit, wherein the first layer is coupled to an electrode array network with a non-conductive material layer, the electrode array network being configured to modulate voltage states at each electrode of the electrode array network to provide a pattern of controlled electric fields for manipulation of the set of sample droplets, the first layer being composed of a flexible hydrophobic material and the second layer being a rigid layer opposite the first layer and displaced from the first layer;
upon activation of a first subset of electrodes of the electrode array network, generating a first stage droplet upon combining the sample droplet with a binding droplet including a set of magnetic particles for binding with targets of the sample droplet;
upon activation of a second subset of electrodes of the electrode array network, transmitting the first stage droplet to a magnetic region between the first layer and the second layer of the magnetic separation unit;
retaining a target portion of the first stage droplet within the magnetic region while transmitting a non-target portion of the first stage droplet to an outlet of the magnetic separation unit, upon activation of a third subset of electrodes of the electrode array network;
upon activation of a fourth subset of electrodes of the electrode array network, generating a second stage droplet upon combining the target portion with an elution droplet for elution of targets from the set of magnetic particles; and
upon activation of a fifth subset of electrodes of the electrode array network, transmitting the target portion of the second stage droplet toward an outlet of the magnetic separation unit.
2 . The method of claim 1 , further including transmitting at least one of the set of droplets across a joint between the magnetic separation unit and a downstream unit of the system.
3 . The method of claim 1 , further including transmitting a droplet of the set of droplets into an open region of the magnetic separation unit and evaporating fluid material from the droplet.
4 . The method of claim 1 , further including transmitting a droplet of the set of droplets into a heating region of the magnetic separation unit.
5 . The method of claim 1 , transmitting a droplet of the set of droplets across a wedge element of the magnetic separation unit and splitting the droplet into multiple sub-portions.
6 . The method of claim 1 , wherein the non-conductive material layer comprises at least one of an oil layer, an adhesive layer, and a resin layer.
7 . The method of claim 1 , further including transmitting a droplet of the set of droplets into a cooling unit and cooling the droplet with the cooling unit, the cooling unit comprising a cooling element proximal to one or more electrodes of the electrode array network.