Compositions and methods for generating massively parallel nucleic acid sequencing libraries
Oligonucleotide primers and methods of use in producing sequencing libraries are provided according to aspects of the present disclosure which include, from 5′ to 3′, a homopolymer-hybridizing region, and an anchor region comprising 5′-(λ) n N m -3′, wherein the homopolymer-hybridizing region is a contiguous sequence of 5 to 20 elements, wherein the elements are nucleotides or T m increasing nucleotide analogs, wherein at least 4 of the elements are T m increasing nucleotide analogs, wherein the homopolymer-hybridizing region hybridizes to a complementary homopolymer tract of a target nucleic acid, wherein the complementary homopolymer tract comprises a contiguous sequence of complementary elements, wherein X is any nucleotide or nucleotide analog with the proviso that λ is not a nucleotide or nucleotide analog complementary to a complementary element of the complementary homopolymer tract, and wherein N is any nucleotide or nucleotide analog.
1. An oligonucleotide primer, comprising, from 5′ to 3′, a 5′ primer tag sequence (PTS) covalently bonded to a 5′ residue of a homopolymer-hybridizing region, wherein the PTS consists of a linker and/or one or more nucleic acid sequences useful in nucleic acid sequence manipulation selected from: a sequencing site, a PCR site, and a hybridization site; the homopolymer-hybridizing region, and an anchor region comprising 5′-(λ) n N m -3′, wherein the homopolymer-hybridizing region hybridizes to a complementary homopolymer tract of a target nucleic acid, wherein the complementary homopolymer tract comprises a contiguous sequence of complementary elements, wherein the homopolymer-hybridizing region, and the anchor region together have a sequence selected from the group consisting of: SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, and SEQ ID NO: 15, and wherein λ is any nucleotide or nucleotide analog with the proviso that k is not a nucleotide or nucleotide analog complementary to a complementary element of the complementary homopolymer tract, and wherein N is any nucleotide or nucleotide analog.
2. An oligonucleotide primer set, comprising two or more oligonucleotide primers according to claim 1 .
3. The oligonucleotide primer set of claim 2 , wherein at least two of the two or more oligonucleotide primers have different anchor regions.
4. The oligonucleotide primer set of claim 2 , comprising at least 12 or more oligonucleotide primers for use where the complementary elements of the target nucleic acid are “A”, wherein the oligonucleotide primer set comprises at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-AA-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-AT-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-AC-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-AG-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-CA-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-CT-3′ at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-CC-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-CG-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-GA-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-GT-3′, at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-GC-3′, and at least one oligonucleotide primer where 5′-(λ) 1 N 1 -3′ is 5′-GG-3′.
5. The oligonucleotide primer set of claim 2 , wherein the oligonucleotide primers of the primer set are provided in an equimolar mixture.
6. A method of generating a sequencing library, comprising:
providing an oligonucleotide primer according to claim 1 ;
annealing the oligonucleotide primer or oligonucleotide primer set to target nucleic acid, the target nucleic acid comprising the complementary homopolymer tract having at least 5 to 20 contiguous complementary elements;
extending the oligonucleotide primer under extension reaction conditions, producing an extension product complementary to at least a portion of the target nucleic acid;
polymerizing a second strand of DNA complementary to the extension product, producing double-stranded nucleic acid; and
amplifying the double-stranded nucleic acid using amplification primers, producing amplicons, thereby introducing one or more primer-derived nucleic acids into the amplicons, the one or more primer-derived nucleic acids comprising at least a sequencing primer binding site sequence.
7. The method of claim 6 , wherein the target nucleic acid is RNA and extending the oligonucleotide primer or primers of the oligonucleotide primer set comprises reverse transcription using a reverse transcriptase to produce a complementary DNA (cDNA).
8. A kit, comprising an oligonucleotide according to claim 1 .