IL-2 muteins and uses thereof
The present application provides for IL-2 muteins, compositions comprising the same, and methods of using the same.
1. A method of increasing T regulatory cell proliferation in a subject, the method comprising administering to the subject an effective amount of a pharmaceutical composition comprising a peptide comprising the amino acid sequence of SEQ ID NO: 27.
2. The method of claim 1 , wherein the peptide further comprises a N-terminal leader peptide having the amino acid sequence of SEQ ID NO: 7.
3. The method of claim 1 , wherein the peptide further comprises a linker peptide at the C-terminus.
4. The method of claim 3 , wherein the linker peptide comprises the sequence of (GGGGS) n , wherein n is 1, 2, 3, or 4.
5. The method of claim 1 , wherein the peptide further comprises a Fc peptide.
6. The method of claim 5 , wherein the Fc peptide comprises the amino acid sequence of SEQ ID NO: 8 or SEQ ID NO: 15.
7. The method of claim 5 , wherein the peptide further comprises a leader peptide having the sequence of SEQ. ID. No: 7 at the N-terminus of the peptide.
8. The method of claim 5 , wherein the Fc peptide is at the C-terminus of the peptide.
9. The method of claim 5 , wherein the Fc peptide is at the N-terminus of the peptide.
10. The method of claim 5 , wherein the peptide further comprises a linker peptide that links the peptide of SEQ ID NO: 27 and the Fc peptide.
11. The method of claim 10 , wherein the peptide further comprises a N-terminal leader peptide comprising the amino acid sequence of SEQ ID NO: 7.
12. The method of claim 10 , wherein the linker peptide is (GGGGS) n , n is 1, 2, 3, or 4.
13. The method of claim 10 , wherein the Fc peptide comprises the sequence of SEQ ID NO: 8 or SEQ ID NO: 15.
14. The method of claim 10 , wherein the Fc peptide is at the C-terminus of the peptide.
15. The method of claim 10 , wherein the Fc peptide is at the N-terminus of the peptide.
16. The method of claim 10 , wherein the peptide comprises an amino acid sequence of SEQ ID NO: 40.
17. A method of activating T regulatory cells in a subject, the method comprising administering to the subject an effective amount of a pharmaceutical composition comprising a peptide comprising an amino acid sequence of SEQ ID NO: 27.
18. The method of claim 17 , wherein the peptide further comprises a Fc peptide.
19. The method of claim 18 , wherein the Fc peptide is at the C-terminus or the N-terminus of the peptide.
20. The method of claim 18 , wherein the peptide comprises an amino acid sequence of SEQ ID NO: 40.