Materials and methods for reducing viscosity of oil
The invention provides environmentally-friendly compositions and methods for reducing the viscosity of crude oil using microorganisms and/or biosurfactants produced by microorganisms.
1. A method for reducing the viscosity of oil, wherein the method comprises contacting the oil with a composition that comprises a fermentation broth in which a biosurfactant-producing yeast was cultivated, said fermentation broth comprising the biosurfactant-producing yeast in inactive form and/or one or more biosurfactants produced by the yeast; wherein the yeast is selected from Starmerella bombicola, Pseudozyma aphidis, Wickerhamomyces anomalus, Pichia sydowiorum, Pichia guilliermondii , and Pichia lynferdii.
2. The method of claim 1 , wherein the yeast is Wickerhamomyces anomalus.
3. The method of claim 1 , wherein the yeast is Pichia guilliermondii.
4. The method of claim 1 , wherein the yeast is Starmerella bombicola.
5. The method of claim 1 , wherein the yeast is Pseudozyma aphidis.
6. The method of claim 1 , wherein the composition comprises a glycolipid biosurfactant selected from sophorolipids (SLPs) and mannosylerythritol lipids (MELs).
7. The method of claim 1 , wherein the composition comprises the one or more biosurfactants without the yeast.
8. The method of claim 1 , wherein the step of contacting the composition with oil comprises injecting the composition into a wellbore, flowline, or oil tank.
9. The method of claim 1 , used to improve oil transmission through an oil field pipe line, tank, casing, tubing, rod, pump, and/or wellbore.
10. The method of claim 1 , used to convert a heavy asphalt portion of crude oil into lower molecular weight compounds.
11. The method of claim 1 , wherein the composition is produced on-site at a distance not more than 50 miles from the site at which it is used.
12. The method of claim 1 , further comprising the step of subjecting the oil to cavitation.
13. A method for recovering oil from oil sands comprising applying a composition that comprises a fermentation broth in which a biosurfactant-producing yeast was cultivated, said fermentation broth comprising an inactive biosurfactant-producing yeast and/or one or more biosurfactants produced by the yeast, to the oil sands; allowing the oil to detach from the sands; and piping out the oil.
14. The method of claim 13 , wherein the method further comprises reducing the viscosity of the oil recovered by applying a composition that comprises a fermentation broth in which a biosurfactant-producing yeast was cultivated, said fermentation broth comprising an inactive biosurfactant-producing yeast and/or one or more biosurfactants produced by the yeast, to the oil, and optionally, subjecting the oil to cavitation.
15. The method, according to claim 13 , wherein the yeast is selected from Starmerella bombicola, Wickerhamomyces anomalus, Pichia sydowiorum, Pichia guilliermondii , and Pichia lynferdii ; and the biosurfactant is selected from sophorolipids (SLPs), and mannosylerythritol lipids (MELs).