Coronavirus vaccine formulations
Disclosed herein are coronavirus Spike (S) proteins and nanoparticles comprising the same, which are suitable for use in vaccines. The nanoparticles present antigens from pathogens surrounded to and associated with a detergent core resulting in enhanced stability and good immunogenicity. Dosages, formulations, and methods for preparing the vaccines and nanoparticles are also disclosed.
1. An immunogenic composition comprising:
(a) a non-naturally occurring sudden acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (CoV S) glycoprotein, wherein the CoV S glycoprotein consists of two modifications compared to a native CoV S glycoprotein wherein
(i) the first modification is an inactivated furin cleavage site consisting of mutation of amino acids 669-672 to QQAQ (SEQ ID NO: 7); and
(ii) the second modification is mutation of amino acids 973 and 974 to proline;
wherein the amino acids are numbered according to SEQ ID NO: 2;
(b) a saponin adjuvant; and
(c) a pharmaceutically acceptable buffer.
2. The immunogenic composition of claim 1 , comprising between about 5 μg and about 25 μg of CoV S glycoprotein.
3. The immunogenic composition of claim 2 , comprising about 5 μg of CoV S glycoprotein.
4. The immunogenic composition of claim 1 , comprising about 3 μg of CoV S glycoprotein.
5. The immunogenic composition of claim 1 , comprising about 50 μg of saponin adjuvant.
6. The immunogenic composition of claim 1 , comprising a non-ionic detergent core, wherein the CoV S glycoprotein and the non-ionic detergent core are in the form of a nanoparticle.
7. The immunogenic composition of claim 6 , wherein the non-ionic detergent is selected from the group consisting of polysorbate-20 (PS20), polysorbate-40 (PS40), polysorbate-60 (PS60), polysorbate-65 (PS65), and polysorbate-80 (PS80).
8. The immunogenic composition of claim 7 , wherein the non-ionic detergent is PS80.
9. The immunogenic composition of claim 1 , wherein the saponin adjuvant comprises:
(i) a first iscom particle comprising fraction A of Quillaja saponaria Molina and not fraction C of Quillaja saponaria Molina; and
(ii) a second iscom particle comprising fraction C of Quillaja saponaria Molina and not fraction A of Quillaja saponaria Molina.
10. The immunogenic composition of claim 9 , wherein fraction A of Quillaja saponaria Molina and fraction C of Quillaja saponaria Molina account for about 85% by weight and about 15% by weight, respectively, of the sum of the weights of fraction A of Quillaja saponaria Molina and fraction C of Quillaja saponaria Molina in the saponin adjuvant.
11. A method of stimulating an immune response against SARS-CoV-2 in a subject comprising administering the immunogenic composition of claim 1 .
12. The method of claim 11 , wherein the immunogenic composition comprises between about 5 μg and about 25 μg of CoV S glycoprotein.
13. The method of claim 12 , wherein the immunogenic composition comprises about 5 μg of CoV S glycoprotein.
14. The method of claim 11 , wherein the immunogenic composition comprises about 3 μg of CoV S glycoprotein.
15. The method of claim 11 , wherein the immunogenic composition comprises about 50 μg of saponin adjuvant.
16. The method of claim 11 , wherein the saponin adjuvant comprises:
(i) a first iscom particle comprising fraction A of Quillaja saponaria Molina and not fraction C of Quillaja saponaria Molina; and
(ii) a second iscom particle comprising fraction C of Quillaja saponaria Molina and not fraction A of Quillaja saponaria Molina.
17. The method of claim 16 , wherein fraction A of Quillaja saponaria Molina and fraction C of Quillaja saponaria Molina account for about 85% by weight and about 15% by weight, respectively, of the sum of the weights of fraction A of Quillaja saponaria Molina and fraction C of Quillaja saponaria Molina in the saponin adjuvant.
18. The method of claim 11 , wherein the immunogenic composition comprises a non-ionic detergent core, wherein the CoV S glycoprotein and the non-ionic detergent core are in the form of a nanoparticle.
19. The method of claim 18 , wherein the non-ionic detergent is selected from the group consisting of polysorbate-20 (PS20), polysorbate-40 (PS40), polysorbate-60 (PS60), polysorbate-65 (PS65), and polysorbate-80 (PS80).
20. The method of claim 19 , wherein the non-ionic detergent is PS80.
21. The method of claim 11 , wherein the subject is administered a first dose at day 0 and a boost dose at day 21.
22. The method of claim 11 , wherein a single dose of the immunogenic composition is administered.
23. The method of claim 11 , comprising administering a second immunogenic composition before or after the immunogenic composition of claim 1 is administered.
24. The method of claim 23 , wherein the second immunogenic compositions is the immunogenic composition of claim 1 .
25. The method of claim 23 , wherein the second immunogenic composition is a different immunogenic composition than the immunogenic composition of claim 1 .
26. The method of claim 25 , wherein the second immunogenic composition is selected from the group consisting of an mRNA encoding a SARS-CoV-2 Spike glycoprotein, a plasmid DNA encoding a SARS-CoV-2 Spike glycoprotein, a viral vector encoding a SARS-CoV-2 Spike glycoprotein, and an inactivated SARS-CoV-2 virus.