Biological fluid filtration system
A biological fluid filtration system for scanning a biological fluid so as to filter potentially undesirable constituents from the biological fluid for therapeutic or diagnostic purposes. The biological fluid filtration system generally includes a fluid receiving device adapted to receive a biological fluid. A valve including an inlet, a first outlet, and a second outlet is fluidly connected to the fluid receiving device. The biological fluid within the fluid receiving device is scanned by a scanner to produce scanned data relating to the biological fluid. A control unit in communication with the scanner and the valve receives the scanned data and controls the valve based on the scanned data. The valve is controlled to direct the biological fluid through either the first or second outlet of the valve depending upon the constituents of the biological fluid identified by the control unit.
1. A method of testing a drug ex vivo on a biological fluid, comprising the steps of: receiving the biological fluid by a fluid filtration device, wherein the fluid filtration device includes an optical scanner comprising a digital holographic microscope, a control unit in communication with the optical scanner, wherein the control unit is configured to receive scanned data from the optical scanner and to compare the scanned data to reference data, wherein the reference data comprise one or more characteristics of a healthy cell; and wherein the control unit is configured to recognize the one or more characteristics of the healthy cell within the scanned data, a first outlet and a second outlet, wherein the fluid filtration device is adapted to receive the biological fluid and generate the scanned data relating to the biological fluid within the fluid receiving device using the optical scanner; separating a first subset of constituents and a second subset of constituents from the biological fluid by the fluid filtration device, wherein the first subset of constituents is determined to include an undesirable constituent using the scanned data; directing the first subset of constituents through the first outlet of the fluid filtration device; directing the second subset of constituents through the second outlet of the fluid filtration device; and testing the drug on the first subset of constituents.
2. The method of claim 1 , further comprising the step of isolating the first subset of constituents ex vivo.
3. The method of claim 1 , further comprising the step of applying genomic profiling to the first subset of constituents.
4. The method of claim 1 , further comprising the step of applying transcriptomic profiling to the first subset of constituents.
5. The method of claim 1 , wherein the undesirable constituent is comprised of an unhealthy cell.
6. The method of claim 5 , wherein the undesirable constituent is comprised of a circulating tumor cell.
7. The method of claim 5 , wherein the undesirable constituent is comprised of a circulating tumor cell cluster.
8. The method of claim 1 , wherein the undesirable constituent is comprised of a pathogen.
9. The method of claim 1 , wherein the second subset of constituents does not include the undesirable constituent.
10. The method of claim 9 , further comprising the step of returning the second subset of constituents to a source of the biological fluid.
11. The method of claim 1 , wherein the drug is comprised of a chemotherapeutic agent.
12. The method of claim 1 , wherein the drug is comprised of an immunotherapy treatment.
13. The method of claim 1 , wherein the biological fluid is comprised of blood.
14. The method of claim 1 , wherein the biological fluid is comprised of a leukapheresis extract.
15. The method of claim 1 , wherein the first outlet of the fluid filtration device is fluidly connected to an isolation channel.
16. The method of claim 15 , wherein the second outlet of the fluid filtration device is fluidly connected to a source of the biological fluid.
17. The method of claim 1 , wherein the fluid filtration device further comprises
a valve comprising an inlet, a first outlet, and a second outlet, wherein the inlet of the valve is fluidly connected to the fluid receiving device, wherein the first outlet of the valve is fluidly connected to the first outlet of the fluid filtration device, and wherein the second outlet of the valve is fluidly connected to the second outlet of the fluid filtration device; and
a control unit in communication with the optical scanner and the valve, wherein the control unit is configured to receive the scanned data from the optical scanner, and wherein the control unit is configured to control the valve based on the scanned data from the optical scanner; wherein the control unit is configured to control the valve to (a) direct the biological fluid including the first subset of constituents through the first outlet if the control unit determines that the scanned data indicates a presence of an undesirable constituent within the biological fluid or (b) direct the biological fluid including the second subset of constituents through the second outlet if the control unit determines that the scanned data does not indicate a presence of an undesirable constituent within the biological fluid.
18. The method of claim 1 , further comprising the step of culturing the first subset of constituents.
19. The method of claim 1 , further comprising the step of performing drug resistance diagnostics on the first subset of constituents.
20. The method of claim 1 , further comprising the step of performing drug sensitivity diagnostics on the first subset of constituents.
21. The method of claim 1 , wherein the optical scanner is configured to scan the biological fluid within the fluid filtration device to produce the scanned data relating to the biological fluid within the fluid filtration device, wherein the scanner comprises a light source and a digital holographic microscope objective.
22. The method of claim 1 , wherein the second subset of constituents comprises healthy cells.