Methods, Systems and Apparatus for Reducing Pathogen Loads in Circulating Body Fluids
A nanocomposition for use in treating a pathogen condition using phthalocyanine dye, such as IR700. A nanocomposition having IR700, an 8PEG nanoparticle and a pathogen targeting peptide. Administering a product comprising IR700 to a patient, whereby the IR700 is delivered to pathogen tissue, and found in only pathogen tissue; and administering light to activate the IR700, thereby producing an ROS.
1 . A nanocomposition, for use in treating a pathogen condition, the nanocomposition comprising:
a photosensitizer (PS) comprising a chlorin, a bacteriochlorin, or a phthalocyanine;
a nanoparticle (NP) comprising 8PEG, 8PEGA, or 8PEGMAL; and
a targeting agent (TA), wherein the targeting agent is a pathogen targeting peptide (PTP);
wherein the nanocomposition is configured for providing a photodynamic therapy for the pathogen condition.
2 . The nanocomposition of claim 1 , wherein the PS is IR700.
3 . The nanocomposition of claim 1 , wherein the nanocomposition has less than 3 PS per NP.
4 . The nanocomposition of claim 1 , wherein nanoparticle comprises an 8PEG nanoparticle, and the 8PEG nanoparticle has at least one free arm.
5 . The nanocomposition of claim 1 , wherein the PS and the TA and are directly bonded to the nanoparticle.
6 . The nanocomposition of claim 1 , wherein the PS and the TA are covalently bonded to the nanoparticle.
7 . The nanocomposition of claim 1 , wherein the PS and TA are both are attached to the nanoparticle by a linking moiety.
8 . The nanocomposition of claim 1 , wherein the nanocomposition has a hydrodynamic diameter selected from the group consisting of 70 nm and less, 50 nm and less, 25 nm and less, and 10 nm and less.
9 . The nanocomposition of claim 1 , wherein the nanoparticle has a mass selected from the group consisting of about 10 kDa and greater, about 20 kDa and greater, about 40 kDa and greater, and about 50 kDa and greater.
10 . A method of treating a pathogen condition comprising:
administering to an animal a nanocomposition comprising:
a photosensitizer (PS) comprising a chlorin, a bacteriochlorin, or a phthalocyanine;
a nanoparticle (NP) comprising 8PEG, 8PEGA, or 8PEGMAL; and
a targeting agent (TA), wherein the targeting agent is a pathogen targeting peptide (PTP); wherein the nanocomposition is configured for providing a photodynamic therapy for the pathogen condition.
waiting a sufficient time for the nanocompositions to accumulate in a targeted pathogen tissue of the animal; and,
illuminating the targeted pathogen tissue with light having a wavelength and sufficient energy to activate the PS, thereby producing reactive oxygen species (ROS) adjacent to the pathogen.
11 . The method of claim 10 , wherein the light is a laser beam.
12 . The method of claim 10 , wherein the reactive oxygen species kill the pathogen forming pathogen fragments that stimulate an immune response against the pathogen.
13 . The method of claim 10 , wherein lasting immunity is provided against the pathogen.
14 . The method of claim 10 , wherein the pathogen is a virus, bacteria, fungi or parasite.
15 . The method of claim 10 wherein the virus is selected from a group containing influenza viruses, corona viruses, SARS-CoV-2, Ebola, HIV, SARS, MERS.
16 . The method of claim 10 wherein the bacteria is selected from a group containing gram-positive and gram-negative bacteria.
17 . The method of claim 10 , wherein the animal is a human.
18 . A method of reducing the pathogen load in a patient comprising:
administering a photodynamic therapy (PDT) composition to the patient; wherein the PDT composition comprises a photoactive agent and a targeting agent that specifically targets the pathogen;
binding the PDT composition to the pathogen in the blood;
removing the patients' blood from the patient and illuminating the removed blood with light to activate the photoactive agent, thereby producing produce reactive oxygen species adjacent to the pathogen;
placing the illuminated blood into the patient, whereby the load for the pathogen in the patient is reduced.
19 . The method of claim 18 , wherein the reactive oxygen species kill the pathogen forming pathogen fragments that stimulate an immune response for the pathogen.
20 . The method of claim 18 , wherein lasting immunity is provided for the pathogen.
21 . A kit comprising:
a container having a plurality of the nanocomposition comprising:
a photosensitizer (PS) comprising a chlorin, a bacteriochlorin, or a phthalocyanine;
a nanoparticle (NP) comprising 8PEG, 8PEGA, or 8PEGMAL; and
a targeting agent (TA), wherein the targeting agent is a pathogen targeting peptide (PTP); wherein the nanocomposition is configured for providing a photodynamic therapy for the pathogen condition; and
an illumination light source having a wavelength and power selected to activate the PS.
22 . The kit of claim 21 , wherein the illumination light comprises a disposable optical delivery device.