System and method for protein corona sensor array for early detection of diseases
The present disclosure provides a system comprising a communication interface and computer for assigning a label to the biomolecule fingerprint, wherein the label corresponds to a biological state. The present disclosure also provides a sensor arrays for detecting biomolecules and methods of use. In some embodiments, the sensor arrays are capable of determining a disease state in a subject.
1. A method of identifying a pattern of protein biomarkers from a biological fluid, wherein the method comprises:
a. incubating a plurality of nanoparticles that differs from each other in at least one physicochemical property with the biological fluid from a subject in a disease state to allow biomolecule coronas to form on surfaces of the plurality of nanoparticles, wherein at least one of the plurality of nanoparticles comprises a magnetic core, and wherein the pattern of protein biomarkers differs based on the different physicochemical properties of the particles;
b. isolating the plurality of nanoparticles and the surface-bound biomolecule coronas; and
c. analyzing biomolecules of the biomolecule coronas by mass spectrometry to identify a relationship between the biomolecule coronas and a protein biomarker in the pattern of protein biomarkers associated with the disease state, wherein the analysis is performed at least in part using a computer.
2. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the plurality of nanoparticles are selected from liposomes, metal nanoparticles, polymeric nanoparticles, and graphene nanoparticles.
3. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein at least one of the plurality of nanoparticles is a liposome.
4. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein at least one of the plurality of nanoparticles is a metal nanoparticle.
5. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the computer comprises a personal computer, laptop, tablet, telephone, Smart phone, or personal digital assistant.
6. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the plurality of nanoparticles with surface-bound biomolecule coronas are isolated from the biological fluid and purified to remove unbound and high abundance biomolecules to allow identification of low abundance biomarkers.
7. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the plurality of nanoparticles with surface-bound biomolecule coronas are isolated from the biological fluid and purified to remove unbound and highly abundant biomolecules by a method comprising liquid chromatography.
8. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein a change in a protein biomarker in response to a therapy administered to the subject is monitored.
9. A method of identifying a protein biomarker from a biological fluid according to claim 8 , wherein the therapy administered to the subject prior to testing is a drug.
10. A method of identifying a protein biomarker from a biological fluid according to claim 8 , wherein the therapy administered to the subject prior to testing is a cancer treatment.
11. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the protein biomarker would not have been detected if the analysis was carried out directly on a biological fluid isolated from the subject.
12. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the disease is cancer.
13. A method of identifying a protein biomarker from a biological fluid according to claim 12 , wherein the disease is lung cancer, melanoma or ovarian cancer.
14. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the plurality of nanoparticles comprises a metal selected from the group consisting of gold and silver.
15. A method of identifying a protein biomarker from a biological fluid according to claim 11 , wherein the biological fluid is plasma.
16. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the magnetic core is superparamagnetic.
17. A method of identifying a protein biomarker from a biological fluid according to claim 1 , wherein the pattern of protein biomarkers comprises at least two biomolecule corona signatures.
18. A method of identifying a protein biomarker from a biological fluid according to claim 17 , wherein the pattern of protein biomarkers comprises a first biomolecule corona signature and a second biomolecule corona signature, wherein the first biomolecule corona signature and the second biomolecule corona signature comprise distinct biomolecules and common biomolecules.
19. A method of identifying a protein biomarker from a biological fluid according to claim 18 , wherein the common biomolecules differ in concentration in the first biomolecule corona signature compared to the second biomolecule corona signature.
20. A method of identifying a protein biomarker from a biological fluid according to claim 19 , wherein (i) a presence or absence of the distinct biomolecules and (ii) the concentration of the common biomolecules in the pattern of protein biomarkers is correlated with the disease state.
21. A method of identifying a protein biomarker from a biological fluid according to claim 20 , wherein the analysis is performed to distinguish between at least two types of disease states.
22. A method of identifying a protein biomarker from a biological fluid according to claim 21 , wherein the analysis is performed to distinguish between at least two types of cancer.