IP Library › Granted Patent US 12,227,798
Granted Patent B2
US 12,227,798 · App. 17/217,354 · Granted Feb 18, 2025

Methods and compositions for incorporating nucleotides

Inventors: Steven Gordon (Weston, MA); Jerzy Olejnik (Brookline, MA)
Assignee: IsoPlexis Corporation
C12Q1/6869C07H19/00C12Q1/6874G01N21/05G01N21/6428G01N21/645C12Q2535/101G01N2021/0346G01N2021/058G01N2021/6417G01N2021/6419G01N2021/6421G01N2021/6439G01N2021/6441G01N2021/6471G01N2201/062G01N2201/0627G01N2201/12
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Quick Facts
Patent No.
US 12,227,798
App. No.
17/217,354
Granted
Feb 18, 2025
Kind
B2
Abstract

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Claims (12)

1. A method for determining an identity of a nucleotide at a position, comprising:

a) providing nucleic acid, polymerase, and a mixture of labeled and non-labeled reversible terminators, said labeled reversible terminators comprising a label linked to a nucleotide analogue;

b) exposing said nucleic acid, said polymerase, and said mixture to extension conditions for 10 minutes or less, wherein a first nucleotide analogue from said mixture is incorporated into said nucleic acid at a position;

c) detecting the label of said incorporated first nucleotide analogue; and

d) determining the identity of the incorporated nucleotide at said position.

2. The method of claim 1 , wherein said exposing to extension conditions is for 2 minutes or less.

3. The method of claim 1 , wherein each reversible terminator contains a reversibly removable chemical moiety capping the 3′-OH group.

4. The method of claim 1 , wherein said reversible terminators comprise four different nucleotide analogues.

5. The method of claim 4 , wherein each of said four different nucleotide analogues comprises a unique label, said label selected from the group consisting of BODIPY, Rhodamine, Carboxyrhodamine, and Cyanine.

6. The method of claim 1 , wherein said reversible terminators are reversibly terminated by a disulfide bond at the 3′ position.

7. The method of claim 1 , wherein said reversible terminators are reversibly terminated by an azidomethyl group at the 3′ position.

8. The method of claim 1 , wherein said nucleic acid comprises primer hybridized to template.

Assignments (6)
RELEASE OF SECURITY INTEREST Recorded Mar 24, 2023
From: PERCEPTIVE CREDIT HOLDINGS III, LP
To: ISOPLEXIS CORPORATION
Reel/Frame 063235/0942 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 25, 2021
From: GORDON, STEVEN; OLEJNIK, JERZY
To: INTELLIGENT BIO-SYSTEMS, INC.
Reel/Frame 057283/0237 →
PATENT PURCHASE AGREEMENT Recorded Aug 25, 2021
From: QIAGEN SCIENCES, LLC
To: ISOPLEXIS CORPORATION
Reel/Frame 057298/0389 →
CHANGE OF NAME Recorded Aug 25, 2021
From: INTELLIGENT BIO-SYSTEMS, INC.
To: QIAGEN WALTHAM, INC.
Reel/Frame 057298/0413 →
MERGER Recorded Aug 25, 2021
From: QIAGEN WALTHAM, INC.
To: QIAGEN SCIENCES, LLC
Reel/Frame 057298/0416 →
SECURITY AGREEMENT Recorded May 28, 2021
From: ISOPLEXIS CORPORATION
To: PERCEPTIVE CREDIT HOLDINGS III, LP
Reel/Frame 056421/0929 →
Continuity (8)
Continuation 16430064 · Jun 3, 2019
Continuation 15911801 · Mar 5, 2018
Continuation 15214737 · Jul 20, 2016
Continuation 14691042 · Apr 20, 2015
Continuation 13305415 · Nov 28, 2011
Continuation 12405779 · Mar 17, 2009
Provisional Application 61037845 · Mar 19, 2008
Related Publication 20210230686A1 · Jul 29, 2021
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