DETECTION OF COLORECTAL CANCER
The present disclosure provides, among other things, methods for colorectal cancer detection (e.g., screening) and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer screening that include analysis of methylation status of one or more methylation biomarkers, and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer detection (e.g., screening) that include detecting (e.g., screening) methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA. In various embodiments, the present disclosure provides methods for colorectal cancer screening that include detecting (e.g., screening) methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA, using MSRE-qPCR.
1 .- 12 . (canceled)
13 . A kit for use in colorectal cancer screening, the kit comprising:
(a) an oligonucleotide primer pair for amplification of a methylation locus within gene ZNF132;
(b) an oligonucleotide primer pair for amplification of a first methylation locus within gene ADAMTS2; and
(c) an oligonucleotide primer pair for amplification of a second methylation locus within gene ADAMTS2.
14 . The kit of claim 13 , further comprising:
(d) an oligonucleotide primer pair for amplification of a methylation locus within gene ZNF542; and
(e) an oligonucleotide primer pair for amplification of a methylation locus within gene LONRF2.
15 . The kit of claim 14 , further comprising:
an oligonucleotide primer pair for amplification of a methylation locus within gene ZNF492.
16 . The kit of claim 13 , wherein (a) is an oligonucleotide primer pair for amplification of ZNF132 '415 (primer pair SEQ ID NO: 91 and SEQ ID NO: 92).
17 . The kit of claim 16 , wherein (b) is an oligonucleotide primer pair for amplification of ADAMTS2 '254 (primer pair SEQ ID NO: 53 and SEQ ID NO: 54).
18 . The kit of claim 17 , wherein (c) is an oligonucleotide primer pair for amplification of ADAMTS2 '284 (primer pair SEQ ID NO: 55 and SEQ ID NO: 56).
19 . The kit of claim 14 , wherein (d) is an oligonucleotide primer pair for amplification of ZNF542 '502 (primer pair SEQ ID NO: 81 and SEQ ID NO: 82).
20 . The kit of claim 14 , wherein (e) is an oligonucleotide primer pair for amplification of LONRF2 '281 (primer pair SEQ ID NO: 49 and SEQ ID NO: 50).
21 . The kit of claim 15 , wherein (f) is an oligonucleotide primer pair for amplification of ZNF492 '069 (primer pair SEQ ID NO: 95 and SEQ ID NO: 96).
22 .- 30 . (canceled)