Methods of epigenetic analysis
The present invention provides for methods of epigenetic analysis. In some cases, the methods may include obtaining a sample comprising a nucleic acid sequence. In some cases, the nucleic acid sequence may comprise one or more epigenetic marks. The methods may include performing a sequencing. The methods may include distinguishing a hydroxymethylated base from a methylated base.
1. A composition comprising a mixture of a methylcytosine dioxygenase, a DNA glucosyltransferase, and nucleic acid comprising glucosylated 5-hydroxymethylcytosine.
2. The composition of claim 1 , wherein said methylcytosine dioxygenase is a ten eleven translocation (TET) enzyme.
3. The composition of claim 2 , wherein said TET enzyme comprises TET1, TET2, TET3, CXXC4, or a combination thereof.
4. The composition of claim 1 , wherein said DNA glucosyltransferase is a DNA glucosyltransferase encoded by a bacteriophage of the T even family.
5. The composition of claim 1 , wherein said DNA glucosyltransferase is a beta-glucosyltransferase.
6. The composition of claim 1 , wherein said DNA glucosyltransferase is an alpha-glucosyltransferase.
7. The composition of claim 1 , wherein said mixture comprises a DNA methyltransferase.
8. The composition of claim 7 , wherein said DNA methyltransferase is DNMT1.
9. The composition of claim 1 , further comprising a glucose donor substrate.
10. The composition of claim 9 , wherein said glucose donor substrate comprises uridine diphosphate glucose (UDPG).
11. The composition of claim 1 , further comprising a buffer.
12. The composition of claim 1 , further comprising 5-methylcytosine.