Bioartificial vascular pancreas
The present invention provides compositions, systems and methods for treating diabetes in a subject. The composition of the present invention includes a decellularized vascular graft, a biocompatible hydrogel encasement with tunable rigidity, and a plurality of cells such as pancreatic islet cells.
1. A composition comprising:
an acellular vascular graft having a lumen that is adapted and configured to be in direct contact with a perfusate passing through it;
a biocompatible hydrogel encasement, wherein the biocompatible hydrogel encasement surrounds an outer surface of the acellular vascular graft; and,
a plurality of cells, wherein the plurality of cells comprises pancreatic islet cells;
wherein the plurality of cells are:
a. seeded on a surface of the biocompatible_hydrogel encasement and/or
b. seeded within the biocompatible hydrogel encasement.
2. The composition of claim 1 , wherein the acellular vascular graft comprises a decellularized arterial graft.
3. The composition of claim 1 , wherein the acellular vascular graft comprises a decellularized venous graft.
4. The composition of claim 1 , wherein the acellular vascular graft comprises a decellularized engineered vascular graft.
5. The composition of claim 1 , wherein the biocompatible hydrogel encasement comprises fibrin, fibrinogen, thrombin, collagen, elastin, gelatin, chitosans, alginate, laminin, hyaluronans, silk, polyethylene glycol, isolated extracellular matrix hydrogels, or combinations thereof.
6. The composition of claim 1 , wherein the plurality of cells are selected from the group consisting of: alpha cells, beta cells, delta cells, PP cells, epsilon cells, insulinoma cells, transgenic cells, knock-out cells, knock-in cells, or otherwise genetically modified cells, embryonic stem cells (ESCs), induced pluripotent stem cells (IPSCs), and combinations thereof.
7. The composition of claim 1 , wherein the pancreatic islet cells are mammalian pancreatic islet cells selected from the group consisting of bovine, porcine, murine, rattus, equine, and human islet cells.