ENRICHMENT SUPPLEMENT FOR ANTIMICROBAL MATRICES
Salts of Fe (II), Zn (II), or both used in methods for enriching food matrices containing antimicrobial compounds. A kit comprising salts of Fe (II), Zn (II) useful for such methods.
1 . A method of growing of a target microorganism comprising incubating a matrix having one or more antimicrobial compounds that are active against the target microorganism in the presence of an Fe(II) salt or a Zn(II) salt.
2 . The method of claim 1 , wherein the matrix is a food matrix.
3 . The method of claim 1 , wherein the matrix comprises a nut matrix, a food matrix, or a combination thereof.
4 . The method of claim 1 , wherein the target microorganism is Listeria or Salmonella.
5 . The method of claim 1 , wherein the microorganism is neither a yeast nor an iron oxidizing or reducing microorganism.
6 . The method of claim 1 , further comprising dissolving or dispersing the matrix in a culture broth containing an enrichment medium.
7 . The method of any of the claim 6 , further comprising admixing the matrix, the culture broth, and the Fe (II) or Zn (II) salt, wherein the Fe(II) salt or Zn(II) salt is at a concentration of 100 micromolar or greater.
8 . The method of any of the claim 6 , further comprising admixing the matrix, the culture broth, and the Fe (II) or Zn (II) salt, wherein the Fe(II) salt or Zn(II) salt is at a concentration of 1,000 micromolar or less.
9 . The method of claim 7 , wherein the enrichment medium is a selective enrichment medium for the target microorganism.
10 . The method of claim 1 , wherein the Zn (II) salt is in the form of zinc chloride.
11 . The method of claim 1 , wherein the Fe (II) salt is in the form of ferrous sulfate.
12 . The method of claim 1 , further comprising detecting the presence of a target microorganism.
13 . A kit comprising:
enrichment media that is selective for Salmonella or Listeria ; and
a Zn (II) salt or an Fe (II) salt.
14 . The kit of claim 13 , wherein the Zn (II) salt or an Fe (II) salt is a component of the enrichment media.
15 . The kit of claim 13 , wherein the Zn (II) salt or Fe (II) salt is separate from the enrichment media.
16 . The method of claim 8 , wherein the enrichment medium is a selective enrichment medium for the target microorganism.
17 . The method of claim 4 , wherein the enrichment medium is a selective enrichment medium for the target microorganism.