Method for stratifying the risk of BK virus nephropathy after a kidney transplant
The present application describes and claims a novel method for identifying and stratifying the risk of developing a BK virus nephropathy (“Nx BK-v” below) in patients having undergone a kidney transplant. This method uses an index combining at least three bio-markers: i) the intensity of the anti-BK-v memory T lymphocyte response (memory T lymphocytes which are specific to BK-v, hereinafter “LTm anti-BK-v”), ii) the number of occurrences of incompatibility in the HLA alleles of class I and class II between the donor and the recipient of the graft, taking into account iii) the viral charge of the BK-v virus in the whole blood of the patient. The present method allows a very precise evaluation of the risk of developing an Nx BK-v during the months following the test with the aim of optimising the immuno-suppressive treatment in order to better preserve the transplanted kidney.
1 . A method for assessing the risk of developing a BK virus (BKV) nephropathy in a patient who had kidney transplantation from a kidney donor, said method comprising the following steps:
a) measuring the viral load of BKV in a biological sample of said patient,
b) measuring the normalized intensity of the CD4 + or CD8 + memory T cell response specific for BKV in a biological sample from said patient, said normalization being performed relative to the BKV viral load measured in step a), and
c) determining the number of HLA allele differences between the kidney donor and said patient,
wherein a high risk of developing BKV nephropathy is concluded when:
in relation with a viral load of 10 3 copies of BK virus per mL of blood,
the normalized intensity of the CD4 + or CD8 + memory T cell response specific for BKV is less than or equal to 100 intensity measurement units, and
the number of differences between said patient and the donor of the transplanted kidney is less than or equal to 5.
2 . The method according to claim 1 , wherein the three parameters a), b) and c) are then compiled to create a stratification index for the risk that the patient will develop BK virus nephropathy.
3 . The method according to claim 1 , wherein the BKV viral load is measured in step a) by quantitative PCR from a sample of whole blood or plasma from said patient.
4 . The method according to claim 1 , wherein the response of the BKV specific CD4 + or CD8 + memory T cells measured in step b) is determined by contacting peripheral blood mononuclear cells from said patient with BKV peptides, and by assessing the proliferation of T cells present in said mononuclear cells after 4 to 7 days of culture.
5 . The method according to claim 1 , wherein the response of the BKV specific CD4 + or CD8 + memory T cells measured in step b) is determined by contacting peripheral blood mononuclear cells from said patient with BKV peptides, and by assessing the proliferation of T cells present in said mononuclear cells after 4 to 7 days of culture by determining the proportion of CD4 + T cells having diluted a proliferation marker after 4 to 7 days of culture in the presence of said peptides.
6 . The method according to claim 1 , wherein the response of the BKV specific CD4 + or CD8 + memory T cells measured in step b) is determined by contacting peripheral blood mononuclear cells from said patient with BKV peptides, and by assessing the proliferation of T cells present in said mononuclear cells after 4 to 7 days of culture by determining the proportion of CD8 + T cells having diluted a proliferation marker after 4 to 7 days of culture in the presence of said peptides.
7 . The method according to claim 1 , wherein a low risk of developing BK virus (BKV) nephropathy is concluded when:
a) in relation with a viral load of 10 3 copies of BK virus per mL of blood,
b) the normalized intensity of the CD4 + or CD8 + memory T cell response specific for BKV is greater than 100 intensity measurement units, and
c) the number of differences between said patient and the transplant donor is greater than 5.
8 . An in vitro method for assessing the response of a kidney transplant patient to blood replication of BKV comprising providing said patient immunosuppressive treatment, modifying said patient's immunosuppressive treatment, and then performing the method of claim 1 repeatedly at regular time intervals after said kidney transplant.
9 . The method according to claim 1 , wherein said method is an in vitro method for assessing the response of a kidney transplant patient to blood replication of BKV after modification of immunosuppressive treatment, and wherein said method is repeated before and after each treatment modification.
10 . The method according to claim 1 , wherein said method is an in vitro method for assessing the response of a kidney transplant patient to blood replication of BKV after modification of immunosuppressive treatment, and wherein said method is repeated approximately every 3 to 6 months.
11 . A kit containing:
a) reagents for conducting BK virus quantitative PCR, and
b) reagents for conducting the immunological method described in claim 4 on whole blood.
12 . The kit according to claim 11 , wherein the reagents b) contain BKV peptide sequences or polyclonal lymphocyte stimulation peptide sequences and, optionally, lymphocyte labeling reagents.
13 . The kit according to claim 11 containing:
a) specific primers for amplifying portions of the BKV genome, and
b) BKV peptide sequences, and, optionally
c) the reagents necessary for conducting quantitative PCR with DNA extraction and/or
d) the reagents for lymphocyte labeling.