Methods and compositions for promoting wound healing in a subject suffering from ectodermal dysplasias
The present invention relates to a method for promoting wound healing in a subject suffering from Ectodermal dysplasia in need thereof comprising a step of administering subcutaneously, intradermally or topically to said subject a therapeutically effective amount of a compound which restores the activity of p63. Inventors have performed a primary culture of patient keratinocytes suffering from ectodermal dysplasias with two compounds which restore the activity of p63 (e.g. STIMA-1 and/or PRIMA-1Met). They have shown that there is an important differentiation of the keratinocytes of said patient compared to the cells not treated with these compounds. They observed that the activity of p63 mutated is restored, thus the proliferation and differentiation of keratinocytes from the patient are activated. Moreover, inventors have used PRIMA-1Met by topical application on a young patient suffering from ectodermal dysplasias and shown that said patient presents an improvement on her hand. Typically, severe skin erosions (on hands and feet) are healing when PRIMA-1Met is administered topically on the hand.
1 . A method for promoting healing of a wound in a subject suffering from ectodermal dysplasia (ED) comprising a step of administering to said subject a therapeutically effective amount of at least one compound which restores the activity of p63, wherein the ectodermal dysplasia is ankyloblepharon-ectodermal defects-cleft lip/palate syndrome (AEC), wherein the at least one compound is PRIMA-1 Met , and wherein the ED affects at least one cornea.
2 . The method of claim 1 , wherein the at least one compound is formulated as an ophthalmic drop or an ophthalmic ointment.
3 . The method of claim 1 , wherein the administering step is performed during a vascular phase of wound healing.
4 . The method according to claim 1 , wherein the PRIMA-1 Met restores the proliferation and development of epithelial cells and epidermal cells.
5 . The method according to claim 1 , wherein the at least one compound is formulated as a cream, an ointment, or gel.
6 . The method according to claim 1 , wherein the wounds are caused by skin erosion and/or a scalp infection.
7 . The method according to claim 1 , wherein the method prevents and/or treats pain caused by the wounds.
8 . The method according to claim 1 , wherein the mutation is not T533P.