MUSCLE-TARGETING COMPLEXES AND USES THEREOF IN TREATING MUSCLE ATROPHY
Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload inhibits activity of a pro-atrophy gene. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide or RNAi oligonucleotide.
1 .- 115 . (canceled)
116 . An anti-transferrin receptor antibody, wherein the antibody comprises a lysine residue covalently linked via a linker comprising a cleavable valine-citrulline sequence to a molecular payload configured for modulating a pro-atrophy gene, wherein the antibody binds in the range of C89 to F760 of human transferrin receptor protein 1 (TfR1) having an amino acid sequence as set forth in SEQ ID NO: 1, and wherein the antibody comprises:
a heavy chain variable region (VH) comprising a heavy chain complementarity determining region 1 (CDR-H1), a heavy chain complementarity determining region 2 (CDR-H2), and a heavy chain complementarity determining region 3 (CDR-H3); and
a light chain variable region (VL) comprising a light chain complementarity determining region 1 (CDR-L1), a light chain complementarity determining region 2 (CDR-L2), and a light chain complementarity determining region 3 (CDR-L3).
117 . The anti-transferrin receptor antibody of claim 116 , wherein the molecular payload is a small molecule or a peptide.
118 . The anti-transferrin receptor antibody of claim 116 , wherein the anti-transferrin receptor antibody is in the form of a Fab fragment, Fab′ fragment, F(ab′) 2 fragment, ScFv, or Fv fragment.
119 . A complex comprising an anti-transferrin receptor antibody covalently linked to an oligonucleotide that targets a pro-atrophy gene,
wherein the oligonucleotide is 15 to 35 nucleotides in length and comprises a region of complementarity to the pro-atrophy gene, wherein the region of complementarity is at least 12 nucleotides in length; and
wherein the anti-transferrin receptor antibody binds in the range of C89 to F760 of human transferrin receptor protein 1 (TfR1) having an amino acid sequence as set forth in SEQ ID NO: 1.
120 . The complex of claim 119 , wherein the anti-transferrin receptor antibody is in the form of a ScFv, Fab fragment, Fab′ fragment, F(ab′) 2 fragment, or Fv fragment.
121 . The complex of claim 119 , wherein the anti-transferrin receptor antibody binds human TfR1 with a K D of 10 −11 M to 10 −6 M.
122 . The complex of claim 119 , wherein the anti-transferrin receptor antibody is a humanized antibody.
123 . The complex of claim 119 , wherein the anti-transferrin receptor is covalently linked to the oligonucleotide via a cleavable linker.
124 . The complex of claim 123 , wherein the cleavable linker comprises a valine-citrulline sequence.
125 . The complex of claim 119 , wherein the anti-transferrin receptor antibody is covalently linked to the oligonucleotide via conjugation to a lysine residue or a cysteine residue of the anti-transferrin receptor antibody.
126 . The complex of claim 119 , wherein the complex is configured to promote transferrin receptor mediated internalization of the oligonucleotide into a muscle cell.
127 . A method of modulating expression level of a pro-atrophy gene in a muscle cell, the method comprising contacting the muscle cell with the complex of claim 119 .
128 . A method of treating muscle atrophy in a subject, the method comprising administering to the subject a complex comprising an anti-transferrin receptor antibody covalently linked to an oligonucleotide that targets a pro-atrophy gene,
wherein the oligonucleotide is 15 to 35 nucleotides in length and comprises a region of complementarity to the pro-atrophy gene, wherein the region of complementarity is at least 12 nucleotides in length; and
wherein the anti-transferrin receptor antibody binds in the range of C89 to F760 of human transferrin receptor protein 1 (TfR1) having an amino acid sequence as set forth in SEQ ID NO: 1.