Genetic regions and genes associated with increased yield in plants
The present invention relates to methods and compositions for identifying, selecting and/or producing a plant or germplasm having root increased drought tolerance and/or increased yield under non-drought conditions as compared to a control plant. A maize plant, part thereof and/or germplasm, including any progeny and/or seeds derived from a maize plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided.
1. A method of selecting a first maize plant or germplasm that displays either increased yield under drought or increased yield under non-drought conditions, the method comprising:
a) isolating nucleic acids from the first maize plant or germplasm;
b) detecting in the first maize plant or germplasm at least one allele of a quantitative trait locus that is associated with increased yield under drought or non-drought conditions, wherein said allele is located within 10 cM, 5 CM or 1 cM from yield QTL 6 which comprises an A at position 501 of SEQ ID NO:6, which corresponds to Chromosome 9 comprising an A allele at position 135348128;
c) selecting said first maize plant or germplasm, or selecting a progeny of said first maize plant or germplasm, comprising at least one allele associated with increased yield under drought or non-drought conditions; and,
d) crossing said selected first maize plant or germplasm with a second maize plant or germplasm to produce an introgressed maize plant or germplasm comprising said QTL 6, wherein the introgressed maize plant or germplasm displays increased yield under drought and non-drought conditions as compared to a maize plant or germplasm not comprising said QTL 6.
2. The method of claim 1 , wherein the at least one allele is detected using a composition comprising a detectable label.
3. A method introgressing a water optimization locus into a maize plant, said method comprising:
a) isolating a nucleic acid from a first population of maize plants;
b) detecting in said nucleic acid the presence of at least one allele of a quantitative trait locus that is associated with increased yield under drought conditions, wherein said allele is located within 10 cM, 5 cM or 1 cM from yield QTL 6, and wherein said QTL6 comprise an A at position 501 of SEQ ID NO:6 which corresponds to Chromosome 9 comprising an A allele at position 135348128;
c) selecting one or more plants with the water optimization locus from the first population of maize plants; and
d) producing offspring from the one or more plants with the water optimization locus selected in c) by crossing the selected plants with a second maize population not having the water optimization locus, wherein the offspring exhibit improved water optimization as compared to the first population.
4. The method of claim 3 , wherein the at least one allele is detected by the genetic marker of SEQ ID NO: 6.
5. The method of claim 3 , wherein the maize plant belongs to the non-stiff stalk heterotic group.
6. The method of claim 3 , wherein said marker is associated with the presence of a water optimization gene comprising SEQ ID NO: 17 and a gene encoding a protein comprising SEQ ID NO: 24.
7. The method of claim 3 , wherein the maize plant belongs to the stiff stalk heterotic group.
8. The method of claim 3 , wherein the maize plant is a hybrid maize plant and/or an elite maize plant.
9. A plant having introduced into its genome a water optimization gene and a yield allele wherein said water optimization gene comprises a nucleotide sequence encoding at least one polypeptide comprising SEQ ID NO: 24 and the yield allele is SEQ ID NO: 6.
10. The plant of claim 9 , wherein said plant has increased yield as compared to a control plant.
11. The plant of claim 10 , wherein increased yield is yield under water deficit conditions.
12. The method of claim 1 , wherein a parental line of said first maize plant was selected by or identified by a nucleotide probe or primer that annealed to SEQ ID NO: 6 and said parental line conferred increased yield as compared to a plant not comprising SEQ ID NO: 6.
13. The plant of claim 9 , wherein said gene is introduced by heterologous expression.
14. The plant of claim 9 , wherein said gene is introduced by gene editing.
15. The plant of claim 9 wherein said gene is introduced by breeding or trait introgression.
16. The plant of claim 9 , wherein said plant is maize.
17. The plant of claim 16 , wherein said plant is an elite maize line or a hybrid.