Wheat CENH3 alleles
The present invention relates to wheat plants comprising a mutation causing an alteration of the amino acid sequence in centromere histone H3 (CENH3), which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the wheat plants of the present invention and haploid and doubled haploid wheat plants obtainable by crossing the wheat plants of the present invention with wildtype wheat plants.
1. A wheat plant comprising at least an A genome, a B genome, and a D genome, wherein the B genome comprises a knock-out mutation in a CENH3α gene, and wherein the D genome comprises a knock-out mutation in a CENH3α gene, and further wherein the A genome comprises a mutated CENH3α gene comprising at least one knock-down mutation at a 5′ splice site of an intron.
2. The wheat plant of claim 1 , wherein the knock-down mutation is a restored frame shift mutation or a large deletion mutation.
3. The wheat plant of claim 1 , wherein the wheat plant is homozygous for a knock-out mutation in a CENH3α gene in the B genome.
4. The wheat plant of claim 1 , wherein the wheat plant is biallelic for a knock-out mutation in a CENH3α gene in the B genome.
5. The wheat plant of claim 1 , wherein the wheat plant is homozygous for a knock-out mutation in a CENH3α gene in the D genome.
6. The wheat plant of claim 1 , wherein the wheat plant is biallelic for a knock-out mutation in a CENH3α gene in the D genome.
7. The wheat plant of claim 1 , wherein the wheat plant is homozygous, biallelic, or a combination thereof for a knock-out mutation in a CENH3α gene in the B genome and the D genome.
8. The wheat plant of claim 2 , wherein the wheat plant is homozygous for the restored frame shift CENH3α mutation.
9. The wheat plant of claim 2 , wherein the wheat plant is heterozygous for the restored frame shift CENH3α mutation.
10. The wheat plant of claim 1 , wherein the wheat plant is homozygous for a knock-down mutation in a CENH3α gene of the A genome and homozygous for a knock-out mutation in a CENH3α gene in the B genome and the D genome.
11. The wheat plant of claim 10 , wherein the knock-down mutation in a CENH3α gene of the A genome comprises a sequence selected from the group consisting of SEQ ID NOs: 69, 70, and 71.