IP Library › Granted Patent US 12,290,055
Granted Patent B2
US 12,290,055 · App. 17/293,415 · Granted May 6, 2025

Transgenic betta

Inventors: Alan Blake (Austin, TX); Richard Crockett (Wilton, CT); Aidas Nasevicius (Tampa, FL)
Assignee: GloFish, LLC
A01K67/0275C12N15/8509A01K2227/40A01K2267/01
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Quick Facts
Patent No.
US 12,290,055
App. No.
17/293,415
Granted
May 6, 2025
Kind
B2
Abstract

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Claims (21)

1. A transgenic Betta comprising a chromosomally integrated expression cassette encoding a green, red or orange fluorescent protein, wherein the Betta comprises a transformation event from sperm selected from a group consisting of a “Green Betta 1 transformation event”, an “Orange Betta 1 transformation event and a “Red Betta 1 transformation event”, sperm exhibiting the Green Betta 1 transformation event having been deposited at the ATCC as deposit number PTA-126548, sperm exhibiting the Orange Betta 1 transformation event having been deposited at the ATCC as deposit number PTA-126549, and sperm exhibiting the Red Betta 1 transformation event having been deposited at the ATCC as deposit number PTA-126550.

2. The transgenic Betta of claim 1 , further defined as a fertile, transgenic Betta.

3. The transgenic Betta of claim 1 , wherein the fish is homozygous for the integrated expression cassette.

4. The transgenic Betta of claim 1 , wherein the fish is heterozygous for the integrated expression cassette.

5. A method of providing a transgenic Betta to the ornamental fish market, comprising obtaining a transgenic Betta in accordance with claim 1 , and distributing the fish to the ornamental fish market.

6. The method of claim 5 , wherein the fish are distributed by a grower to a commercial distributor.

7. The method of claim 5 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.

8. The method of claim 7 , wherein the retailer is a multi-product retailer having an ornamental fish department.

9. A method of producing a transgenic Betta comprising: (a) obtaining a Betta that comprises a chromosomally integrated expression cassette encoding a green, red or orange fluorescent protein in accordance with claim 1 ; and (b) breeding the obtained Betta with a second Betta to provide a transgenic Betta, wherein the transgenic Betta comprises the transformation event.

10. The method of claim 9 , wherein the second Betta is a non-transgenic Betta.

11. A progeny of a transgenic Betta of claim 1 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the progeny Betta comprises a transformation event selected from a group consisting of a “Green Betta 1 transformation event”, an “Orange Betta 1 transformation event”, a “Red Betta 1 transformation event”,.

12. The progeny fish of claim 11 , further defined as a fertile, transgenic Betta.

13. The progeny fish of claim 11 , wherein the progeny Betta is homozygous for the integrated expression cassette.

14. The progeny fish of claim 11 , wherein the progeny Betta is heterozygous for the integrated expression cassette.

15. A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish in accordance with claim 11 , and distributing the fish to the ornamental fish market.

16. The method of claim 15 , wherein the fish are distributed by a grower to a commercial distributor.

17. The method of claim 16 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.

18. The method of claim 17 , wherein the retailer is a multi-product retailer having an ornamental fish department.

19. A method of producing a transgenic fish comprising: (a) obtaining a transgenic fish in accordance with claim 11 ; and (b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the transformation event.

20. The method of claim 19 , wherein the second fish is a non-transgenic fish.

21. Cell lines for making a transgenic Betta comprising: cells comprising the Green Betta 1 transformation event deposited at the ATCC as deposit number PTA-126548, cells comprising the Orange Betta 1 transformation event deposited at the ATCC as deposit number PTA-126549, and cells comprising the Red Betta 1 transformation event deposited at the ATCC as deposit number PTA-126550.

Assignments (2)
SECURITY INTEREST Recorded Apr 23, 2024
From: SPECTRUM BRANDS, INC.; GLOFISH, LLC; SPECTRUM BRANDS PET GROUP, INC.; SPECTRUM BRANDS PET LLC; EMPOWER BRANDS, LLC; UNITED INDUSTRIES CORPORATION
To: ROYAL BANK OF CANADA
Reel/Frame 067201/0653 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 25, 2022
From: BLAKE, ALAN; CROCKETT, RICHARD; NASEVICIUS, AIDAS
To: GLOFISH, LLC
Reel/Frame 058761/0420 →
Continuity (6)
Provisional Application 62760480 · Nov 13, 2018
Provisional Application 62760445 · Nov 13, 2018
Provisional Application 62760464 · Nov 13, 2018
Provisional Application 62760498 · Nov 13, 2018
Provisional Application 62760489 · Nov 13, 2018
Related Publication 20220022432A1 · Jan 27, 2022
References Cited (96)
US 7135613B1 · Gong et al. · 2006 [cited by applicant]
US 7355095B2 · Tsai et al. · 2008 [cited by applicant]
US 7700825B2 · Blake et al. · 2010 [cited by applicant]
US 7834239B2 · Gong et al. · 2010 [cited by applicant]
US 8232450B1 · Blake et al. · 2012 [cited by applicant]
US 8232451B1 · Blake et al. · 2012 [cited by applicant]
US 8378169B2 · Gong et al. · 2013 [cited by applicant]
US 8581025B2 · Blake et al. · 2013 [cited by applicant]
US 9968077B2 · Blake et al. · 2018 [cited by applicant]
US 10798923B2 · Blake et al. · 2020 [cited by applicant]
US 20020178461A1 · Lin · 2002 [cited by applicant]
US 20030162292A1 · Tsai et al. · 2003 [cited by applicant]
US 20040117866A1 · Tsai · 2004 [cited by applicant]
US 20040143864A1 · Gong et al. · 2004 [cited by applicant]
US 20050198701A1 · Lian et al. · 2005 [cited by applicant]
US 20050273874A1 · Tsai et al. · 2005 [cited by applicant]
US 20080052787A1 · Gong et al. · 2008 [cited by applicant]
US 20090025645A1 · Blake et al. · 2009 [cited by applicant]
US 20090035788A1 · Griesbeck et al. · 2009 [cited by applicant]
US 20090133138A1 · Tsai · 2009 [cited by applicant]
US 20090255006A1 · Dougan et al. · 2009 [cited by applicant]
US 20100037330A1 · Siripattarappavat et al. · 2010 [cited by applicant]
US 20100037331A1 · Blake et al. · 2010 [cited by applicant]
US 20100050280A1 · Blake et al. · 2010 [cited by applicant]
US 20100145889A1 · Blake et al. · 2010 [cited by applicant]
US 20120210453A1 · Blake et al. · 2012 [cited by applicant]
US 20120317665A1 · Blake et al. · 2012 [cited by applicant]
US 20130133093A1 · Blake et al. · 2013 [cited by applicant]
US 20130333060A1 · Blake et al. · 2013 [cited by applicant]
US 20140033338A1 · Blake et al. · 2014 [cited by applicant]
US 20140130195A1 · Blake et al. · 2014 [cited by applicant]
US 20150216148A1 · Blake et al. · 2015 [cited by applicant]
US 20150216149A1 · Blake et al. · 2015 [cited by applicant]
US 20150216150A1 · Blake et al. · 2015 [cited by applicant]
US 20150216151A1 · Blake et al. · 2015 [cited by applicant]
US 20160128310A1 · Blake · 2016 [cited by examiner]
US 20170258057A1 · Blake et al. · 2017 [cited by applicant]
US 20200113159A1 · Blake et al. · 2020 [cited by applicant]
US 20200396972A1 · Blake et al. · 2020 [cited by applicant]
US 20210051927A1 · Blake et al. · 2021 [cited by applicant]
US 20220090126A1 · Blake et al. · 2022 [cited by applicant]
CN 1590548 · 2005 [cited by applicant]
CN 103540611A · 2014 [cited by applicant]
CN 106070063A · 2016 [cited by applicant]
EP 2166107 · 2010 [cited by applicant]
JP 2004321173A · 2004 [cited by applicant]
WO WO2000049150 · 2000 [cited by applicant]
WO WO2008022208 · 2008 [cited by applicant]
WO WO2009148549 · 2009 [cited by applicant]
WO 2018183728A1 · 2018 [cited by applicant]
Zhu Z. et al., Novel gene transfer into the fertilized eggs of gold fish ( [cited by applicant]
Du S.J et al., “Growth enhancement in transgenic atlantic salmon by the use of an “All Fish” chimeric growth hormone gene contrust”, Bio/Technology, Nature Publishing Group, vol. 10: 176-181 (1992). [cited by applicant]
Khoo H.W. et al., “Sperm cells as vectors for introducing foreign DNA into ebrafish”, Aquaculture, 107, issue 1: 1-19 (1992). [cited by applicant]
Sin F.Y.T. et al., Gene transer in chinook salmon ( [cited by applicant]
Zelenin A.V. et al., “The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles”, FEBS Lett. 287(1-2): 118-120 (1991). [cited by applicant]
Szelei J. et al., “Liposome-mediated gene transfer in fish embryos”, Transgenic Research 3: 116-119 (1994). [cited by applicant]
Xu Y. et al., “Fast Skeletal Muscle-Specific Expression of a Zebrafish Myosin Light Chain 2 Gene and Characterization of Its Promoter by Direct Injection into Skeletal Muscle”, DNA and Cell Biology, vol. 18: 85-95 (1999… [cited by applicant]
Chourrout D. et al., “High efficiency gene transfer in rainbow trout ( [cited by applicant]
Penman D.J. et al., “Factors Affecting Survival and Integration Following Microinjection of Novel DNA into Rainbor Trout Eggs”, Aquaculture, 85: 35-50 (1990). [cited by applicant]
Brem G. et al., Gene Transfer in Tilapia ( [cited by applicant]
Gross M.L. et al., “Molecular analysis and growth evaluation of northern pike ( [cited by applicant]
Devlin R.H. et al., “Extraordinary salmon growth”, Scientific Correspondence, Nature, vol. 371: 209-210 (1994). [cited by applicant]
Tsai H.J. et al., Electroporation of sperm to introduce foreign DNA into the genome of loach ( [cited by applicant]
Shagin et al., “GFP-like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity”, Molecular Biology and Evolution, vol. 21(5): 841-850 (2004). [cited by applicant]
C. Walker and G. Streisinger, Freezing Sperm in Zebrafish Book—A guide for the Laboratory Use of Zebrafish ( [cited by applicant]
Draper et al., “A High-Throughput Method for Zebrafish Sperm Cryopreservation and In Vitro Fertilization”, Journal of Visualized Experiments, Jove, 29, e1395: 1-5 (2009). [cited by applicant]
Vick B.M. et al., “Learning the scientific method using GloFish”, Zebrafish, vol. 9(4): 226-241 (2012). [cited by applicant]
International Search Report and Written Opinion for PCT/US2018/025224, mailed Jul. 6, 2018. [cited by applicant]
International Search Report and Written Opinion for PCT/US2019/013072, mailed Apr. 26, 2019. [cited by applicant]
International Search Report and Written Opinion for PCT/US2019/061155, mailed Mar. 13, 2020. [cited by applicant]
International Search Report and Written Opinion for PCT/US2020/013102, mailed Jun. 19, 2020. [cited by applicant]
Berquand et al., “Analysis of Cytoskeleton-Destabilizing Agents by Optimized Optical Navigation and AFM Force Measurements,” [cited by applicant]
Day et al., “Fluorescent protein tools for studying protein dynamics in living cells: a review,” [cited by applicant]
Finley et al., “Three-color imaging using fluorescent proteins in living zebrafish embryos,” [cited by applicant]
Franco et al., “Control of initial endothelial spreading by topographic activation of focal adhesion kinase,” [cited by applicant]
Gong et al., “Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle,” [cited by applicant]
Gong et al., “Green fluorescent protein (GFP) transgenic fish and their applications,” [cited by applicant]
Ju et al., “Recapitulation of fast skeletal muscle development in zebrafish by transgenic expression of GFP under the mylz2 promoter,” [cited by applicant]
Laranjeira et al., “Glial cells in the mouse enteric nervous system can undergo neurogenesis in response to injury,” [cited by applicant]
Liu et al., “Development of expression vectors for transgenic fish,” [cited by applicant]
Liu et al., “Isolation and characterization of beta-actin gene of carp ( [cited by applicant]
Martynov et al., “Alternative cyclization in GFP-like proteins family,” [cited by applicant]
Nowotschin et al., “Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives,” [cited by applicant]
Parichy et al., “Zebrafish hybrids suggest genetic mechanisms for pigment pattern diversification in [cited by applicant]
Shcherbo et al., “Bright far-red fluorescent protein for whole-body imaging,” [cited by applicant]
Shkrob et al., “Far-red fluorescent proteins evolved from a blue chromoprotein from [cited by applicant]
Stewart, “Go with the glow: fluorescent proteins to light transgenic organisms,” [cited by applicant]
Subach et al., “Conversion of red fluorescent protein into a bright blue probe,” [cited by applicant]
Urbani, “Multi-Color approach to track Salmonella during infection,” [cited by applicant]
Wan et al., “Generation of two-color transgenic zebrafish using the green and red fluorescent protein reporter genes gfp and rfp,” [cited by applicant]
Zhu et al., “Regulation of the Imo2 promoter during hematopoietic and vascular development in zebrafish,” [cited by applicant]
Zhu et al., “Use of the DsRed fluorescent reporter in zebrafish,” [cited by applicant]
Design U.S. Appl. No. 29/501,874 entitled “Bright Red Fluorescent Tetra” by Alan Blake et al., filed Sep. 9, 2014. [cited by applicant]
Design U.S. Appl. No. 29/501,878 entitled “Bright Blue Fluorescent Tetra” by Alan Blake et al., filed Sep. 9, 2014. [cited by applicant]
Fenner, The Rainbow, Redfin and Albino Minnow sharks [cited by applicant]
Wikipedia description of rainbow shark (2022) (Year: 2022). [cited by applicant]