IP Library Patent Application 17293867
Patent Application
App. No. 17/293,867

METHOD OF PRESERVING CELLS FOR THERAPEUTIC USE

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Patent No.
US None
App. No.
17/293,867
Abstract

Disclosed is a composition including, in a physiologically acceptable medium: a) at least one saccharide; b) at least one vitamin; c) at least one amino acid; d) at least one antioxidant; and e) cells for therapeutic use. The composition has a pH between 7.0 and 8.5, inclusive, and preferably between 7.0 and 8.3. Also disclosed is a method of preserving a sample of cells for therapeutic use, including at least one step of mixing the sample of cells for therapeutic use with the ingredients a) to d) above and a physiologically acceptable medium, the composition that is obtained having a pH between 7.0 and 8.5, inclusive, and preferably between 7.0 and 8.3.

Claims (42)

1 . A composition comprising, in a physiologically acceptable medium:

a) at least one saccharide;

b) at least one vitamin;

c) at least one amino acid;

d) at least one antioxidant, and

e) cells for therapeutic use;

said composition having a pH of between 7.0 and 8.5.

2 . The composition according to claim 1 , wherein it has a pH between 7.0 and 8.3.

3 . The composition according to claim 1 , wherein it comprises a platelet lysate.

4 . The composition according to claim 1 , wherein the saccharide is selected from among monosaccharides, disaccharides and trisaccharides.

5 . The composition according to claim 1 , wherein the vitamin is selected from among the vitamins B1, B2, B4, B5, B6, B7, B9, PP and the mixtures thereof.

6 . The composition according to claim 1 , wherein the amino acid is selected from among glutamine, alanyl-glutamine, tryptophan, lysine, methionine, phenylalanine, threonine, valine, leucine and isoleucine, arginine, histidine, tyrosine, cysteine and the mixtures thereof.

7 . The composition according to claim 1 , wherein the amino acid is cysteine.

8 . The composition according to claim 1 , wherein the antioxidant is selected from among glutathione, vitamin C, vitamin E, vitamin A, L-cysteine, or the coenzyme Q10.

9 . The composition according to claim 1 , wherein the antioxidant is glutathione.

10 . The composition according to claim 1 , wherein it comprises at least one bicarbonate salt.

11 . The composition according to claim 1 , wherein the cells for therapeutic use are selected from among: natural killer (NK) cells; monocytes; B lymphocytes; T lymphocytes, whether natural or genetically modified; myoblasts; hematopoietic stem cells; mesenchymal stem cells; cardiac cells; and fibroblasts.

12 . The composition according to claim 1 , wherein the physiologically acceptable medium is an aqueous medium containing electrolytes, and wherein the composition comprises:

a) glucose;

b) a mixture of vitamins B1, B2, B4, B5, B6, B7, B9 and PP;

c) a mixture of glutamine, alanyl-glutamine, tryptophan, lysine, methionine, phenylalanine, threonine, valine, leucine, isoleucine, arginine, histidine, tyrosine and cysteine;

d) glutathione; and

e) cells for therapeutic use.

13 . A cell preservation method for preserving a sample of cells for therapeutic use, comprising at least one mixing step of mixing the sample of cells for therapeutic use with:

a) at least one saccharide;

b) at least one vitamin;

c) at least one amino acid;

d) at least one antioxidant; and

a physiologically acceptable medium;

the composition thus obtained having a pH between 7.0 and 8.5.

14 . The cell preservation method according to claim 13 , wherein the pH is between 7.0 and 8.3.

15 . A method for preserving at least one sample of cells for therapeutic use, comprising mixing said sample of cells with a composition comprising, in a physiologically acceptable medium:

a) at least one saccharide;

b) at least one vitamin;

c) at least one amino acid;

d) glutathione;

and having a pH of between 7.0 and 8.5.

16 . The method according to claim 15 , said composition having a pH of between 7.0 and 8.3.

17 . The composition according to claim 2 , wherein it comprises a platelet lysate.

18 . The composition according to claim 2 , wherein the saccharide is selected from among monosaccharides, disaccharides and trisaccharides.

19 . The composition according to claim 3 , wherein the saccharide is selected from among monosaccharides, disaccharides and trisaccharides.

20 . The composition according to claim 2 , wherein the vitamin is selected from among the vitamins B1, B2, B4, B5, B6, B7, B9, PP and the mixtures thereof.

Assignments (3)
CHANGE OF OWNER/APPLICANT'S ADDRESS Recorded Apr 3, 2023
From: LABORATOIRE FRANÇAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
To: LABORATOIRE FRANÇAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
Reel/Frame 063237/0439 →
CHANGE OF OWNER/APPLICANT'S ADDRESS Recorded Sep 21, 2022
From: LABORATOIRE FRANÇAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
To: LABORATOIRE FRANÇAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
Reel/Frame 061493/0885 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 31, 2022
From: DE LARICHAUDY, JOFFREY; CAZALON NEMORIN, SANDY
To: LABORATOIRE FRANÇAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
Reel/Frame 060053/0583 →