Expression of novel cell tags
Disclosed herein are polynucleotides encoding cell tags for use in immunotherapeutic applications, and systems comprising polynucleotide cell tags for regulating the activity of a cell. The compositions, methods and systems described herein provide tools for regulating activity of genetically engineered cells in a subject.
1. A recombinant polypeptide comprising an amino acid sequence having at least 90% identity with the sequence of any one of SEQ ID NOs: 57, 73, 77, and 81;
wherein the recombinant polypeptide does not comprise an intracellular signaling domain.
2. The recombinant polypeptide of claim 1 , comprising an amino acid sequence having at least 90% identity with the sequence of SEQ ID NO: 57.
3. The recombinant polypeptide of claim 1 , comprising an amino acid sequence having at least 95% identity with the sequence of SEQ ID NO: 57.
4. The recombinant polypeptide of claim 1 , comprising an amino acid sequence having at least 99% identity with the sequence of SEQ ID NO: 57.
5. The recombinant polypeptide of claim 1 , further comprising a signal peptide.
6. The recombinant polypeptide of claim 5 , wherein the signal peptide is a GMCSFRα, Ig Kappa, Immunoglobulin E, CD8α, TVB2, CD52, LNGFR signal peptide.
7. The recombinant polypeptide of claim 5 , wherein the signal peptide comprises an amino acid sequence having at least 95% identity with any one of SEQ ID NOs: 2, 4, 6, 8, 10, 12, and 14.
8. The recombinant polypeptide of claim 5 , wherein the signal peptide comprises the amino acid sequence of any one of SEQ ID NOs: 2, 4, 6, 8, 10, 12, and 14.
9. The recombinant polypeptide of claim 5 , wherein the signal peptide comprises an amino acid sequence having at least 95% identity with SEQ ID NO: 4.
10. The recombinant polypeptide of claim 5 , wherein the signal peptide comprises an amino acid sequence having at least 99% identity with SEQ ID NO: 4.
11. The recombinant polypeptide of claim 5 , wherein the signal peptide comprises the amino acid sequence of SEQ ID NO: 4.
12. The recombinant polypeptide of claim 1 , comprising an amino acid sequence having at least 90% identity with any one of SEQ ID NOs: 73, 77, and 81.
13. The recombinant polypeptide of claim 1 , comprising an amino acid sequence having at least 95% identity with any one of SEQ ID NOs: 73, 77, and 81.
14. The recombinant polypeptide of claim 1 , comprising an amino acid sequence having at least 99% identity with any one of SEQ ID NOs: 73, 77, and 81.
15. The recombinant polypeptide of claim 1 , comprising an amino acid sequence of any one of SEQ ID NOs: 73, 77, and 81.