IP Library Granted Patent US 11,555,995
Granted Patent B2
US 11,555,995 · App. 17/324,173 · Granted Jan 17, 2023

Microscope system, control method, and recording medium

Inventors: Motohiro Shibata (Tokyo, JP); Takehiko Hayashi (Tokyo, JP); Keita Kojima (Tokyo, JP); Keigo Mori (Tokyo, JP)
Assignee: Evident Corporation
G02B21/365G02B21/025G02B21/34G06V20/693G01N21/6458G01N2021/6439
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Quick Facts
Patent No.
US 11,555,995
App. No.
17/324,173
Granted
Jan 17, 2023
Kind
B2
Abstract

A microscope system is provided with a microscope that acquires images at least at a first magnification and a second magnification higher than the first magnification, and a processor. The processor is configured to specify a type of a container in which a specimen is placed, and when starting observation of the specimen placed in the container at the second magnification, the processor is configured to specify an observation start position by performing object detection according to the type of container on a first image that includes the container acquired by the microscope at the first magnification, and control a relative position of the microscope with respect to the specimen such that the observation start position is contained in a field of view at the second magnification of the microscope.

Claims (61)

1. A microscope system comprising:

a microscope that acquires images at least at a first magnification and a second magnification higher than the first magnification; and

a processor,

wherein:

the processor is configured to specify a type of a container in which a specimen is placed, and

when starting observation of the specimen placed in the container at the second magnification, the processor is further configured to:

specify an observation start position by performing object detection according to the type of container on a first image that includes the container acquired by the microscope at the first magnification, and

control a relative position of the microscope with respect to the specimen such that the observation start position is contained in a field of view at the second magnification of the microscope.

2. The microscope system according to claim 1 , wherein the processor is configured to:

detect a structure corresponding to the type of the container based on the first image, and

specify the observation start position based at least on a result of detecting the structure.

3. The microscope system according to claim 2 , wherein the processor is configured to:

detect the specimen placed in the container based on the first image, and

specify the observation start position based on the result of detecting the structure and a result of detecting the specimen.

4. The microscope system according to claim 3 , wherein the structure corresponding to the type of the container is (i) a cover slip in a case where the type of the container is a microscope slide, (ii) a hole part provided in a dish in a case where the type of the container is the dish, and (iii) a well in a case where the type of the container is a multi-well plate.

5. The microscope system according to claim 2 , wherein the structure corresponding to the type of the container is (i) a cover slip in a case where the type of the container is a microscope slide, (ii) a hole part provided in a dish in a case where the type of the container is the dish, and (iii) a well in a case where the type of the container is a multi-well plate.

6. The microscope system according to claim 2 , wherein the processor is configured to, in a case where the type of the container is a microscope slide:

specify the observation start position from inside a cover slip overlapping the microscope slide, and

control the relative position such that the observation start position specified from inside the cover slip is contained in the field of view at the second magnification.

7. The microscope system according to claim 2 , wherein the processor is configured to, in a case where the type of the container is a dish:

specify the observation start position from inside the dish, and

control the relative position such that the observation start position specified from inside the dish is contained in the field of view at the second magnification.

8. The microscope system according to claim 2 , wherein the processor is configured to, in a case where the type of the container is a dish:

specify the observation start position from inside a hole part provided in the dish, and

control the relative position such that the observation start position specified from inside the hole part is contained in the field of view at the second magnification.

9. The microscope system according to claim 2 , wherein the processor is configured to, in a case where the type of the container is a multi-well plate:

specify the observation start position from inside a well provided in the multi-well plate, and

control the relative position such that the observation start position specified from inside the well is contained in the field of view at the second magnification.

10. The microscope system according to claim 2 , wherein the processor is configured to:

detect a staining state of the specimen placed in the container based on the first image, and

change a setting of an observation method of the microscope to an observation method corresponding to a result of detecting the staining state.

11. The microscope system according to claim 1 , wherein the processor is configured to, in a case where the type of the container is a microscope slide:

specify the observation start position from inside a cover slip overlapping the microscope slide, and

control the relative position such that the observation start position specified from inside the cover slip is contained in the field of view at the second magnification.

12. The microscope system according to claim 1 , wherein the processor is configured to, in a case where the type of the container is a dish:

specify the observation start position from inside the dish, and

control the relative position such that the observation start position specified from inside the dish is contained in the field of view at the second magnification.

13. The microscope system according to claim 1 , wherein the processor is configured to, in a case where the type of the container is a dish:

specify the observation start position from inside a hole part provided in the dish, and

control the relative position such that the observation start position specified from inside the hole part is contained in the field of view at the second magnification.

14. The microscope system according to claim 1 , wherein the processor is configured to, in a case where the type of the container is a multi-well plate:

specify the observation start position from inside a well provided in the multi-well plate, and

control the relative position such that the observation start position specified from inside the well is contained in the field of view at the second magnification.

15. The microscope system according to claim 1 , wherein the processor is configured to:

detect a staining state of the specimen placed in the container based on the first image, and

change a setting of an observation method of the microscope to an observation method corresponding to a result of detecting the staining state.

16. The microscope system according to claim 1 , further comprising:

a detection unit that outputs identification information identifying the type of the container by detecting an identification structure provided in a container holder that holds the container or in the container,

wherein the processor is configured to specify the type of the container based on the identification information outputted by the detection unit.

17. The microscope system according to claim 16 , wherein:

the identification structure is a magnet, and

the detection unit is a magnetic sensor.

18. The microscope system according to claim 1 , wherein the processor is configured to specify the type of the container based on the first image.

19. A control method, executed by a computer, of a microscope system provided with a microscope that acquires images at least at a first magnification and a second magnification higher than the first magnification, the control method comprising:

specifying a type of a container in which a specimen is placed;

when starting observation of the specimen placed in the container at the second magnification, specifying an observation start position according to the type of the container by performing object detection according to the type of container on a first image that includes the container acquired by the microscope at the first magnification; and

controlling a relative position of the microscope with respect to the specimen such that the observation start position is contained in a field of view at the second magnification of the microscope.

20. A non-transitory recording medium storing a program, the program causing a computer of a microscope system provided with a microscope that acquires images at least at a first magnification and a second magnification higher than the first magnification to execute processes comprising:

specifying a type of a container in which a specimen is placed;

when starting observation of the specimen placed in the container at the second magnification, specifying an observation start position according to the type of the container by performing object detection according to the type of container on a first image that includes the container acquired by the microscope at the first magnification, and

controlling a relative position of the microscope with respect to the specimen such that the observation start position is contained in a field of view at the second magnification of the microscope.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 7, 2022
From: OLYMPUS CORPORATION
To: EVIDENT CORPORATION
Reel/Frame 061008/0214 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 12, 2021
From: SHIBATA, MOTOHIRO; HAYASHI, TAKEHIKO; KOJIMA, KEITA; MORI, KEIGO
To: OLYMPUS CORPORATION
Reel/Frame 057769/0819 →
Priority Claims (1)
JP JP2020-088466 · May 20, 2020 · national
Continuity (1)
Related Publication 20210364774A1 · Nov 25, 2021