Methods and systems for monomer chain formation
View Patent ↗Aspects relate to systems and methods for high-contrast cleavage detection. An exemplary method includes a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent, a waveguide configured to propagate an electromagnetic (EM) wave, wherein the waveguide includes a surface upon which the plurality of probes are immobilized, wherein the surface is configured to provide communication between the EM wave and the high-contrast agent, a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent and the plurality of probes are configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte, and a sensor in communication with the waveguide and configured to detect cleaving of the at least a probe by way of the EM wave.
1. A method of monomer chain formation comprising:
immobilizing, using a surface of a waveguide, a monomer chain, wherein the surface of the waveguide further comprises a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent;
providing a first monomer, wherein the first monomer comprises a capping agent;
attaching the first monomer to the monomer chain; and
detecting, using a sensor in communication with the waveguide, presence of the first monomer, wherein detecting further comprises:
introducing a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent is configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte; and
introducing a secondary cleaving agent configured to selectively cleave at least an additional probe as a result of the secondary cleaving agent being in the presence of the analyte.
2. The method of claim 1 , further comprising removing the capping agent from the first monomer.
3. The method of claim 2 , further comprising:
providing a second monomer; and
attaching the second monomer to the monomer chain.
4. The method of claim 1 , wherein the plurality of probes further comprises a plurality of ribonucleic acid (RNA) probes.
5. The method of claim 1 , wherein the analyte comprises the first monomer.
6. The method of claim 1 , wherein the analyte further comprises the capping agent.
7. The method of claim 1 , wherein the waveguide is configured to provide communication between an EM wave and the high-contrast agent by propagating an evanescent wave from the surface.
8. The method of claim 1 , wherein the surface is further configured to provide optical communication between an EM wave and the high-contrast agent.
9. The method of claim 1 , wherein the high-contrast agent is attached to the monomer, and the method further comprises:
detecting the high-contrast agent; and
confirming attachment of the monomer as a function of the detection.
10. The method of claim 1 , further comprising:
emitting, using a light source, a light having a wavelength; and
catalyzing, using the light, bonding of the monomer.
11. The method of claim 1 , wherein the capping agent further comprises the high-contrast agent attached to the capping agent, and the method further comprises:
detecting the high-contrast agent; and
confirming attachment of the monomer as a function of the detection.
12. The method of claim 1 , wherein the primary cleaving agent comprises a CRISPR enzyme.
13. The method of claim 1 , further comprising flowing, using a microfluidic channel, a solution comprising the analyte.
14. The method of claim 1 , wherein attaching the first monomer comprises catalyzing a reaction between the first monomer and the monomer chain.
15. The method of claim 14 , wherein catalyzing the reaction comprises irradiating, using the waveguide, the monomer chain using an electromagnetic radiation.
16. The method of claim 1 , further comprising removing the capping agent by irradiating, using the waveguide, the monomer chain using an electromagnetic radiation.
17. The method of claim 1 , wherein the monomer comprises an amino acid.
18. The method of claim 1 , wherein the monomer comprises a nucleic acid.