Diagnostic methods, therapeutic agents and uses thereof
The present invention provides a method for diagnosing a disease or disorder selected from the group consisting of insulin resistance, a metabolic disorder, diabetes and pre-diabetes in a subject. The method comprises the step of determining the level of a compound represented by structural formula (VI): or a salt thereof. Compositions and method of making thereof are also described.
1. A compound represented by the following formula:
or a salt thereof.
2. The compound of claim 1 , wherein the compound is an ammonium salt of the compound of formula (VII).
3. The compound of claim 1 , wherein the compound is radiolabeled with a radiolabel selected from the group consisting of tritium ( 3 H) and carbon 14 ( 14 C).
4. The compound of claim 3 , wherein the radiolabel is 14 C.
5. A kit comprising an internal standard for determining the level of a compound represented by the following formula:
wherein the internal standard is represented by the following formula:
or a salt thereof.
6. The kit of claim 5 , wherein the internal standard is radiolabeled.
7. The kit of claim 6 , wherein the radiolabel is tritium ( 3 H) or carbon 14 ( 14 C).
8. The kit of claim 5 , further comprising instructions for diagnosing and/or monitoring a disease or disorder selected from the group consisting of insulin resistance, a metabolic disorder, diabetes and pre-diabetes in a subject based on the level of the compound detected in a biological sample from the subject.
9. The kit of claim 8 , wherein the kit further comprises one or more additional biomarkers, wherein the additional biomarkers are related to the disease or disorder.
10. The kit of claim 9 , wherein the one or more additional biomarkers are selected from the group consisting of 2-hydroxybutyrate, linoleoyl lysophosphatidylcholine, oleate, 4-methyl-2-oxo-pentanoate, panthothenate, beta-hydroxybutyrate, and serine.
11. The kit of claim 10 , wherein the kit further comprises 2-hydroxybutyrate and linoleoyl lysophosphatidylcholine as the additional biomarkers.
12. The kit of claim 11 , wherein the kit further comprises one or more additional biomarkers selected from the group consisting 3-methyl-2-oxo-butyric acid, alpha-ketoglutarate, creatine, glycine, isoleucine, leucine, leucine, oleoyl lysophosphatidylcholine, phenylalanine, trigonelline, tyrosine, valine, hydrocinnamic acid, xanthine, mannose, 3-methyl-2-oxovalerate, glycerolphosphorylcholine, adrenate, 3-methyl-2-oxo-pentanoate, 2-methylsuccinate, 1-octadecanol, 2-aminoadipate, 3-hydroxyisobutyrate, alpha-tocopherol, arginine, betaine, decanoylcarnitine, docosatetraenoic acid, glutamic acid, linoleic acid, linolenic acid, margaric acid, N-acetylglycine, octanoylcarnitine, palmitate, palmitoleic acid, palmitoyl lysophosphatidylcholine, stearate, threonine, and tryptophan.