Adeno-associated virus antibodies and fragments thereof
The present disclosure relates to an isolated anti-AAV (adeno-associated virus) antibody or an antigen-binding fragment thereof capable of specifically binding an epitope of AAVrh74 capsid protein and uses thereof.
1. An isolated anti-AAV (adeno-associated virus) antibody or antigen-binding fragment thereof capable of specifically binding an epitope of an AAV capsid protein, wherein the epitope comprises an amino acid sequence QGAGKDNVDYSS (SEQ ID NO: 45); and wherein the antibody or antigen binding fragment thereof comprises:
a. a heavy chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 1; and a light chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 2;
b. a heavy chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 5; and a light chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 6;
c. a heavy chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 13; and a light chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 14;
d. a heavy chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 9; and a light chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 10; or
e. a heavy chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 17; and a light chain variable region comprising an amino acid sequence that is identical to the amino acid sequence set forth in SEQ ID NO: 18.
2. The isolated antibody or antigen binding fragment thereof of claim 1 , wherein the antibody binds to a capsid protein of AAV8 and/or AAV9 with less affinity as compared to a capsid protein of AAVrh74.
3. The isolated antibody or antigen binding fragment thereof of claim 1 , wherein the isolated antibody or antigen binding fragment thereof is labeled with a radioactive, enzymatic, or fluorescent group.
4. The isolated antibody or antigen-binding fragment thereof of claim 1 , wherein the antibody is a full-length antibody or an antibody fragment selected from the group consisting of Fab, Fab′, Fab′-SH, Fd, Fv, dAb, F(ab′)2, scFv, bispecific single chain Fv dimers, diabodies, triabodies, and sxFv genetically fused to the same or a different antibody.
5. The isolated antibody or antigen binding fragment thereof of claim 1 , wherein the antibody is a murine antibody, a chimeric murine/human antibody, a human antibody, an engineered antibody, or a humanized antibody.
6. The isolated antibody of claim 5 , wherein the antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 21 and a light chain comprising the amino acid sequence set forth in SEQ ID NO: 22.
7. The isolated antibody of claim 5 , wherein the antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 23 and a light chain comprising the amino acid sequence set forth in SEQ ID NO: 24.
8. The isolated antibody of claim 5 , wherein the antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 25 and a light chain comprising the amino acid sequence set forth in SEQ ID NO: 26.
9. The isolated antibody of claim 5 , wherein the antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 27 and a light chain comprising the amino acid sequence set forth in SEQ ID NO: 28.
10. A method of detecting a pre-existing antibody against an AAV capsid in a sample from a subject, comprising subjecting the sample to an assay, wherein the antibody or antigen binding fragment thereof of claim 1 is used as a positive control.
11. The method of claim 10 , wherein the assay is an immunoassay.
12. The method of claim 10 , wherein the assay is an immunofluorescence assay, an immunohistochemical assay, a Western blot, a direct enzyme-linked immunosorbent assay (ELISA), an indirect ELISA, a sandwich ELISA, a competitive ELISA, a reverse ELISA, a chemiluminescence assay, a radioimmunoassay, or an immunoprecipitation assay.
13. The method of claim 10 , wherein the assay is an ElectroChemiLuminiescence Immunoassay (ECLIA).
14. The method of claim 10 , further comprising quantifying the pre-existing antibody through an absorbance reading.