MULTISIGNAL REAGENTS FOR LABELING ANALYTES
Provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
1 . A method for providing an oligonucleotide labeled at each of the 5′ end and the 3′ end of the oligonucleotide, comprising the steps of:
providing an DNA oligonucleotide labeled with a first member of a first binding pair at its 5′ end;
adding deoxynucleotides labeled with a first member of a second binding pair to the 3′ end of the oligonucleotide using terminal transferase.
2 . The method of claim 1 , wherein the first member of the first binding pair and the first member of the second binding pair are the same.
3 . The method of claim 1 , wherein the first member of the first binding pair and the first member of the second binding pair are different.
4 . The method of claim 1 , wherein the first member of the first binding pair is streptavidin or avidin.
5 . The method of claim 1 , therein the first member of the second binding pair is digoxigenin.
6 . The method of claim 4 , wherein the first member of the second binding pair is digoxigenin.