TTF Generated Proliferation of Cytotoxic T Cells to Create a Specific Pro-Inflammatory Response
Disclosed are methods of increasing proliferation of CD8+ T cells comprising exposing a target site to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength, wherein the frequency and field strength of the alternating electric field increases proliferation of CD8+ T cells at the target site. Disclosed are methods of generating a pro-inflammatory response in a target site comprising exposing the target site to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength, wherein the frequency and field strength of the alternating electric field generates a pro-inflammatory response at the target site. Disclosed are methods of increasing proliferation of CD8+ T cells comprising exposing CD8+ T cells to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength, wherein the frequency and field strength of the alternating electric field increases proliferation of CD8+ T cells. Disclosed are methods of treating a subject in need of a CD8+ T cell response comprising applying an alternating electric field to a target site of the subject for a period of time, the alternating electric field having a frequency and field strength, wherein the target site comprises one or more CD8+ T cells.
1 . A method of increasing proliferation of CD8+ T cells comprising
exposing a target site to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength,
wherein the frequency and field strength of the alternating electric field increases proliferation of CD8+ T cells at the target site.
2 . A method of generating a pro-inflammatory response in a target site comprising
exposing the target site to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength,
wherein the frequency and field strength of the alternating electric field generates a pro-inflammatory response at the target site.
3 . The method of claim 2 , wherein the pro-inflammatory response comprises one or more cytokines.
4 . The method of claim 3 , wherein the one or more cytokines is IFN-γ or TNF-α.
5 . The method of claim 1 , wherein the target site comprises stimulated CD8+ T cells.
6 . The method of claim 1 , wherein the target site is a site of inflammation or a site comprising a draining lymph node.
7 . The method of claim 6 , wherein the site of inflammation is a burn site, an infection site, or an amputation site.
8 . The method of claim 7 , wherein the infection site is a viral infection site.
9 . The method of claim 8 , wherein the viral infection site is a Pneumonia viral infection site, Meningitis viral infection site, or Herpes zoster viral infection site.
10 . The method of claim 6 , wherein the draining lymph node is near or adjacent to a site of inflammation.
11 . The method of claim 6 , wherein the site of a draining lymph node is near or adjacent to a primary tumor.
12 . (canceled)
13 . The method of claim 1 , wherein the alternating electric field has a frequency of 150 kHz, 200 kHz, or 300 kHz.
14 . The method of claim 1 , wherein the period of time is 1-10 days.
15 . (canceled)
16 . A method of increasing proliferation of CD8+ T cells comprising
exposing CD8+ T cells to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength,
wherein the frequency and field strength of the alternating electric field increases proliferation of CD8+ T cells.
17 . The method of claim 16 , wherein the CD8+ T cells are stimulated CD8+ T cells.
18 . The method of claim 16 , wherein the CD8+ T cells are in a subject.
19 . The method of claim 16 , wherein the CD8+ T cells are exposed to the alternating electrical field in vitro.
20 . The method of claim 16 , wherein the CD8+ T cells are exposed to the alternating electric field through a container suitable for cell culture.
21 . The method of claim 16 , further comprising a step of administering the CD8+ T cells exposed to the alternating electric field into a subject.
22 . The method of claim 21 , wherein the subject has an infection, cancer, a burn or has recently undergone an organ amputation.
23 .- 32 . (canceled)