Reduced background immunoassay plates
View Patent ↗The invention provides reduced background substrates, such as multi-well plates, for use in enzyme-linked immunosorbent assays (ELISAs) and other ligand-binding assays, and methods for making and using the same.
1. A method for making a reduced background multi-well microtiter plate for use in ligand-binding assays, comprising the steps of:
providing a multi-well microtiter plate, wherein the inner surface of at least some of the wells is protein-binding;
coating at least part of the inner surface of at least one of the wells that has a protein-binding surface with a low protein-binding coating material; and
removing the coating from only part of the coated surface.
2. The method of claim 1 , wherein the coating is removed from part or all of the bottom of the coated well(s) and at least predominantly not removed from the side wall of said well(s).
3. A method for making a reduced background multi-well microtiter plate for use in ligand-binding assays, comprising the steps of:
providing a multi-well microtiter plate, wherein said plate is composed of a protein-binding material so that the inner surface of at least some of the wells is protein-binding;
adding a liquid comprising a low protein-binding coating material to at least one of the wells that has a protein-binding inner surface to coat the bottom of the well(s) and the side wall of the well(s)s up to a preselected height or at least the substantially to the top with the low protein-binding coating material;
removing the liquid comprising the coating material from the well(s);
adding a solvent to at least one coated well to remove the coating from only part of the coated surface; and
removing the added solvent from the well(s).
4. The method of claim 3 , wherein the coating is removed from part or all of the bottom of the coated well(s) and at least predominantly not removed from the side wall of said well(s).