Apolipoprotein C3 (APOC3) iRNA compositions and methods of use thereof
The present invention relates to RNAi agents, e.g., double stranded RNA (dsRNA) agents, targeting the apolipoprotein C3 gene (APOC3). The invention also relates to methods of using such RNAi agents to inhibit expression of an APOC3 gene and to methods of preventing and treating an APOC3-associated disorder, e.g., hypertriglyceridemia, non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, polycystic ovary syndrome, kidney disease, obesity, type 2 diabetes mellitus (insulin resistance), hypertension, artherosclerosis and pancreatitis.
1 . A method of inhibiting expression of an apolipoprotein C3 (APOC3) gene in a cell, the method comprising contacting the cell with a dsRNA agent,
wherein the dsRNA agent comprises a sense strand and an antisense strand forming a double stranded region,
wherein the sense strand comprises at least 15 contiguous nucleotides differing by no more than 3 nucleotides from the nucleotide sequence sequence of 5′-CUUAAAAGGGACAGUAUUCUA-3′ (SEQ ID NO: 13), and the antisense strand comprises at least 15 contiguous nucleotides from the nucleotide sequence of 5′-UAGAAUACUGUCCCUUUUAAGCC-3′ (SEQ ID NO: 14),
wherein all of the nucleotides of the sense strand and all of the nucleotides of the antisense strand comprise a modification selected from the group consisting of a 2′-O-methyl modification, a 2′-fluoro modification, and a deoxy-modification,
wherein the sense strand comprises 4 2′-fluoro modified nucleotides at nucleotides 7 and 9-11, counting from the 5′-end, and the antisense strand comprises 2 2′-fluoro modified nucleotides at nucleotides 14 and 16, counting from the 5′-end, and 3 2′-deoxy-modified nucleotides at nucleotides 2, 5, and 7, counting from the 5′-end,
wherein both the sense strand and the antisense strand independently further comprise at least one phosphorothioate or methylphosphonate internucleotide linkage, and
wherein at least one strand is conjugated to a ligand, thereby inhibiting expression of the APOC3 gene in the cell.
2 . The method of claim 1 , wherein the cell is within a subject.
3 . A method of treating a subject having a disorder that would benefit from reduction in apolipoprotein C3 expression, comprising administering to the subject a therapeutically effective amount of a dsRNA agent,
wherein the dsRNA agent comprises a sense strand and an antisense strand forming a double stranded region,
wherein the sense strand comprises at least 15 contiguous nucleotides differing by no more than 3 nucleotides from the nucleotide sequence of 5′-CUUAAAAGGGACAGUAUUCUA-3′ (SEQ ID NO: 13), and the antisense strand comprises at least 15 contiguous nucleotides from the nucleotide sequence of 5′-UAGAAUACUGUCCCUUUUAAGCC-3′ (SEQ ID NO: 14),
wherein all of the nucleotides of the sense strand and all of the nucleotides of the antisense strand comprise a modification selected from the group consisting of a 2′-O-methyl modification, a 2′-fluoro modification, and a deoxy-modification,
wherein the sense strand comprises 4 2′-fluoro modified nucleotides at nucleotides 7 and 9-11, counting from the 5′-end, and the antisense strand comprises 2 2′-fluoro modified nucleotides at nucleotides 14 and 16, counting from the 5′-end, and 3 2′-deoxy-modified nucleotides at nucleotides 2, 5, and 7, counting from the 5′-end,
wherein both the sense strand and the antisense strand independently further comprise at least one phosphorothioate or methylphosphonate internucleotide linkage, and
wherein at least one strand is conjugated to a ligand, thereby treating the subject having the disorder that would benefit from reduction in apolipoprotein C3 expression.
4 . The method of claim 3 , wherein the sense strand comprises two phosphorothioate or methylphosphonate internucleotide linkages at the 5′-terminus.
5 . The method of claim 3 , wherein the antisense strand comprises two phosphorothioate or methylphosphonate internucleotide linkages at both the 5′- and the 3′-terminus.
6 . The method of claim 3 , wherein the sense strand comprises two phosphorothioate or methylphosphonate internucleotide linkages at the 5′-terminus and the antisense strand comprises two phosphorothioate or methylphosphonate internucleotide linkages at both the 5′- and the 3′-terminus.
7 . The method of claim 3 , wherein the ligand is conjugated to the 3′-end of the sense strand.
8 . The method of claim 3 , wherein the ligand is an N-acetylgalactosamine (GalNAc) derivative.
9 . The method of claim 8 , wherein the ligand is one or more GalNAc derivatives attached through a monovalent, bivalent, or trivalent branched linker.
10 . The method of claim 9 , wherein the ligand is
11 . The method of claim 10 , wherein the dsRNA agent is conjugated to the ligand as shown in the following schematic
and, wherein X is O.
12 . A method of treating a subject having a disorder that would benefit from reduction in apolipoprotein C3 expression, comprising administering to the subject a therapeutically effective amount of a dsRNA agent,
wherein the dsRNA agent comprises a sense strand and an antisense strand forming a double stranded region, wherein the sense strand differs by no more than 3 modified nucleotides from the nucleotide sequence of 5′-csusuaaaAfgGfGfAfcaguauucua-3′ (SEQ ID NO: 15) and
wherein the antisense strand differs by no more than 3 modified nucleotides from the nucleotide sequence of 5′-usdAsgadAudAcuguccCfuUfuuaagscsc-3′ (SEQ ID NO: 16),
wherein a, g, c and u are 2′-O-methyl (2′-OMe) A, G, C, and U respectively; Af, Gf, Cf and Uf are 2′-fluoro A, G, C and U respectively; dA is a 2-deoxyadenosine-3-phosphate nucleotide; and s is a phosphorothioate linkage, thereby treating the subject having the disorder that would benefit from reduction in apolipoprotein C3 expression.
13 . The method of claim 12 , wherein the sense strand differs by no more than 2 modified nucleotides from the nucleotide sequence of 5′-csusuaaaAfgGfGfAfcaguauucua-3′ (SEQ ID NO: 15) and wherein the antisense strand differs by no more than 2 modified nucleotides from the nucleotide sequence of 5′-usdAsgadAudAcuguccCfuUfuuaagscsc-3′ (SEQ ID NO: 16).
14 . The method of claim 12 , wherein the sense strand differs by no more than 1 modified nucleotide from the nucleotide sequence of 5′-csusuaaaAfgGfGfAfcaguauucua-3′ (SEQ ID NO: 15) and wherein the antisense strand differs by no more than 1 modified nucleotides from the nucleotide sequence of 5′-usdAsgadAudAcuguccCfuUfuuaagscsc-3′ (SEQ ID NO: 16).
15 . The method of claim 12 , wherein the dsRNA agent is conjugated to a ligand as shown in the following schematic
and, wherein X is O.
16 . A method of treating a subject having a disorder that would benefit from reduction in apolipoprotein C3 expression, comprising administering to the subject a therapeutically effective amount of a dsRNA agent,
wherein the dsRNA agent comprises a sense strand and an antisense strand forming a double stranded region,
wherein the sense strand comprises the nucleotide sequence of 5′-csusuaaaAfgGfGfAfcaguauucua-3′ (SEQ ID NO: 15) and the antisense strand comprises the nucleotide sequence of 5′-usdAsgadAudAcuguccCfuUfuuaagscsc-3′ (SEQ ID NO: 16)),
wherein a, g, c and u are 2′-O-methyl (2′-OMe) A, G, C, and U respectively; Af, Gf, Cf and Uf are 2′-fluoro A, G, C and U respectively; dA is a 2-deoxyadenosine-3-phosphate nucleotide; and s is a phosphorothioate linkage, thereby treating the subject having the disorder that would benefit from reduction in apolipoprotein C3 expression.
17 . The method of claim 16 , wherein the sense strand comprises the nucleotide sequence of 5′-csusuaaaAfgGfGfAfcaguauucua-3′ (SEQ ID NO: 15) and the antisense strand comprises the nucleotide sequence of 5′-usdAsgadAudAcuguccCfuUfuuaagscsc-3′ (SEQ ID NO: 16),
wherein a, g, c and u are 2′-O-methyl (2′-OMe) A, G, C, and U respectively; Af, Gf, Cf and Uf are 2′-fluoro A, G, C and U respectively; dA is a 2-deoxyadenosine-3-phosphate nucleotide; and s is a phosphorothioate linkage; and
wherein the 3′-end of the sense strand is conjugated to a ligand as shown in the following schematic:
wherein X is O, thereby treating the subject having the disorder that would benefit from reduction in apolipoprotein C3 expression.
18 . A method of treating a subject having a disorder that would benefit from reduction in apolipoprotein C3 expression, comprising administering to the subject a therapeutically effective amount of a dsRNA agent,
wherein the dsRNA agent comprises a sense strand and an antisense strand forming a double stranded region,
wherein the sense strand consists of the nucleotide sequence of 5′-csusuaaaAfgGfGfAfcaguauucua-3′ (SEQ ID NO: 15) and the antisense strand consists of the nucleotide sequence of 5′-usdAsgadAudAcuguccCfuUfuuaagscsc-3′ (SEQ ID NO: 16),
wherein a, g, c and u are 2′-O-methyl (2′-OMe) A, G, C, and U respectively; Af, Gf, Cf and Uf are 2′-fluoro A, G, C and U respectively; dA is a 2-deoxyadenosine-3-phosphate nucleotide; and s is a phosphorothioate linkage; and
wherein the 3′-end of the sense strand is conjugated to a ligand as shown in the following schematic:
wherein X is O, thereby treating the subject having the disorder that would benefit from reduction in apolipoprotein C3 expression.
19 . The method of claim 18 , wherein the dsRNA agent is present in a pharmaceutical composition.
20 . The method of claim 18 , wherein the disorder is an apolipoprotein C3-associated disorder.
21 . The method of claim 20 , wherein the apolipoprotein C3-associated disorder is selected from the group consisting of hypertriglyceridemia, non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, polycystic ovary syndrome, kidney disease, obesity, type 2 diabetes mellitus (insulin resistance), hypertension, atherosclerosis and pancreatitis.
22 . The method of claim 20 , wherein the apolipoprotein C3-associated disorder is hypertriglyceridemia.
23 . The method of claim 18 , wherein the subject is human.
24 . The method of claim 18 , wherein the administration of the agent to the subject causes a decrease in hypertriglyceridemia and/or a decrease in APOC3 protein accumulation.
25 . The method of claim 18 , wherein the dsRNA agent is administered to the subject subcutaneously.