TERMINATOR SEQUENCE FOR GENE EXPRESSION IN PLANTS
The present invention discloses polynucleotide sequences that can be used to regulate gene expression in plants. Terminator sequences from Sorghum bicolor that are functional in plants are disclosed.
1 . A recombinant construct comprising a polynucleotide sequence operably linked to a heterologous polynucleotide sequence, wherein the polynucleotide sequence comprises:
(a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:18;
(b) a nucleotide sequence comprising a sequence with at least 95% identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:18; or
(c) a nucleotide sequence comprising a functional fragment of either (a) or (b);
wherein the polynucleotide sequence functions as a transcriptional terminator in a plant cell.
2 . The recombinant construct of claim 1 wherein the polynucleotide is operably linked to a promoter.
3 . A plant comprising the recombinant construct of claim 1 .
4 . The plant of claim 3 wherein the plant is a monocot.
5 . The plant of claim 4 wherein the plant is a maize plant.
6 . A seed comprising the recombinant construct of claim 1 .
7 . The seed of claim 6 wherein the seed is from a monocot plant.
8 . The seed of claim 7 wherein the seed is from a maize plant.
9 . A method of expressing a heterologous polynucleotide in a plant, comprising the steps of:
(a) introducing into a regenerable plant cell the recombinant construct of claim 2 ;
(b) regenerating a transgenic plant from the regenerable plant cell of step (a), wherein the transgenic plant comprises the recombinant construct of claim 2 ; and
(c) obtaining a progeny plant from the transgenic plant of step (b), wherein the progeny plant comprises the recombinant construct of claim 2 and exhibits expression of the heterologous polynucleotide.
10 . The method of claim 9 , wherein the plant is a monocot plant.
11 . The method of claim 10 , wherein the plant is a maize plant.
12 . A plant comprising the recombinant construct of claim 2 .
13 . The plant of claim 12 wherein the plant is a monocot.
14 . The plant of claim 13 wherein the plant is a maize plant.
15 . A seed comprising the recombinant construct of claim 2 .
16 . The seed of claim 15 wherein the seed is from a monocot plant.
17 . The seed of claim 16 wherein the seed is from a maize plant.