Stabilized CRISPR complexes
Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.
1. A CRISPR complex comprising a single guide RNA (sgRNA) covalently bound to a CRISPR effector protein via an adenosine nucleotide or a deoxyadenosine nucleotide at position 22 and a modified uridine nucleotide or a modified deoxyuridine nucleotide at position 49 (the nucleotide positions being numbered consecutively from 5′ to 3′ from nucleotide position 1 at the 5′ end of the target binding region of the sgRNA, wherein the target binding region consists of 20 nucleotides).
2. The CRISPR complex of claim 1 , wherein the CRISPR effector protein is Cas9.
3. The CRISPR complex of claim 1 , wherein the CRISPR complex comprises nuclease activity.
4. A method of editing a target gene in one or more cells comprising administering the CRISPR complex of claim 1 to the one or more cells comprising the target gene, thereby editing the target gene in the one or more cells.
5. A method of preparing the CRISPR complex of claim 1 , the method comprising reacting the sgRNA and the CRISPR effector protein under conditions whereby the unnatural nucleotide of the sgRNA covalently binds to the CRISPR effector protein.
6. A single guide (sgRNA) comprising a crispr RNA (crRNA) region comprising a target binding region and a tracrRNA region that comprises a CRISPR effector protein binding region, wherein the tracrRNA region comprises an unnatural nucleotide that covalently binds to the CRISPR effector protein when the sgRNA binds to the CRISPR effector protein, wherein the sgRNA comprises an adenosine nucleotide or a deoxyadenosine nucleotide at position 22 and a modified uridine nucleotide or a modified deoxyuridine nucleotide at position 49 (the nucleotide positions being numbered consecutively from 5′ to 3′ from nucleotide position 1 at the 5′ end of the target binding region of the sgRNA, wherein the target binding region consists of 20 nucleotides).
7. The sgRNA of claim 6 , wherein the tracrRNA region comprises a CRISPR effector protein binding region for Cas9.
8. A system for preparing a CRISPR complex, the system comprising as components: (i) the sgRNA of claim 6 and (ii) a CRISPR effector protein to which the sgRNA covalently binds.