Granted Patent B2

US 12,281,312 · App. 17/389,878 · Granted Apr 22, 2025

Inventor: Alexander Seifert (Paris, FR)

Assignee: Laboratoire Francais du Fractionnement et des Biotechnologies

C12N15/115C12N15/1048C12N2310/16C12N2320/13C12N2330/30

Abstract

Claims (9)

1. An anti-fibrinogen aptamer that is SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO: 4.

2. An affinity ligand comprising the aptamer according to claim 1 immobilized onto a support.

3. An affinity support comprising a solid support comprising thereon at least one affinity ligand as defined in claim 2 .

4. The affinity support of claim 3 , wherein the solid support is a polymeric gel, filter or membrane.

5. The affinity support of claim 3 , wherein the solid support comprises a polymer selected from the group consisting of agarose, cross-linked agarose, cellulose, polyacrylamide, polyethylene, polyamide, and polysulfone.

6. The affinity ligand of claim 2 , wherein the aptamer is SEQ ID NO: 3.

7. The affinity support of claim 3 , wherein the aptamer is SEQ ID NO: 3.

8. The affinity ligand of claim 2 , wherein the aptamer is SEQ ID NO: 3 comprising a C6 spacer with a terminal amino group at the 5′ end and an inverted deoxy-thymidine at the 3′ end.

9. The affinity ligand of claim 4 , wherein the affinity ligand is grafted onto NHS-activated Sepharose to form an affinity support.

Assignments (2)

Priority Claims (3)

Continuity (2)

References Cited (15)