Endogenous lipase for metal reduction in distillers corn oil
Provided herein are compositions, methods, systems associated with propagation and fermentation, and co-products of biochemical production processes, for example, a DCO co-product resulting from converting oil containing grains into bio chemicals via fermentation in the presence of endogenous esterase. The DCO resulting from the processes exhibits lower metal ion content relative to a DCO obtained in the absence of endogenous fermentation with an esterase such as a lipase. The DCO is useful as a feedstock for the production of renewable diesel.
1. A method of producing DCO, comprising fermenting a ground, grain-based feedstock comprising ground corn in presence of an active lipase,
wherein the DCO produced exhibits at least about a 10% to about 100% reduction in metal ion content or phosphorus content relative to a DCO produced in the absence of the active lipase.
2. The method of claim 1 , wherein the metal ion is selected from the group consisting of Na + , K + , Mg 2+ , and Ca 2+ .
3. The method of claim 1 , wherein the phosphorus content is reduced.
4. The method of claim 1 , further comprising isolating an emulsion comprising DCO; breaking the emulsion comprising the DCO; and isolating the DCO to obtain a renewable diesel feedstock containing less than about 10 ppm metal ions.
5. The method of claim 1 , wherein the active lipase is a triacylglycerol lipase.
6. The method of claim 1 , wherein the DCO produced exhibits at least about a 50% reduction in metal ion content or phosphorus content relative to a DCO produced in the absence of the active lipase.
7. The method of claim 1 , wherein the DCO produced exhibits at least about an 80% reduction in metal ion content or phosphorus content relative to a DCO produced in the absence of the active lipase.