Complex comprising anti-transferrin receptor antibody covalently linked to an oligonucleotide that targets DUX4 RNA
Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload inhibits expression or activity of DUX4. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide or RNAi oligonucleotide.
1. A complex comprising an anti-transferrin receptor antibody covalently linked to an oligonucleotide that targets a DUX4 RNA,
wherein the anti-transferrin receptor antibody binds in the range of C89 to F760 of human transferrin receptor protein 1 (TfR1) having an amino acid sequence as set forth in SEQ ID NO: 1 and wherein the anti-transferrin receptor antibody does not specifically bind to the transferrin binding site of TfR1;
wherein the oligonucleotide is a single-stranded oligonucleotide in the range of 15-35 nucleotides in length, wherein the oligonucleotide comprises a region of complementarity to the nucleotide sequence as set forth in SEQ ID NO: 46, wherein the region of complementarity is at least 15 nucleotides in length, and wherein the oligonucleotide is a phosphorodiamidate morpholino oligomer.
2. The complex of claim 1 , wherein the region of complementarity is at least 20 nucleotides in length.
3. The complex of claim 1 , wherein the oligonucleotide is 100% complementary to the nucleotide sequence of SEQ ID NO: 46.
4. The complex of claim 1 , wherein the oligonucleotide comprises at least 20 consecutive nucleotides of SEQ ID NO: 45.
5. The complex of claim 1 , wherein the oligonucleotide comprises the nucleotide sequence of SEQ ID NO: 45.
6. The complex of claim 1 , wherein the 5′ end of the oligonucleotide is covalently linked to a lysine in the anti-transferrin receptor antibody via a cleavable linker.
7. The complex of claim 6 , wherein the cleavable linker comprises a valine-citrulline sequence.
8. The complex of claim 6 , wherein the complex is obtained by a cycloaddition reaction between an azide and an alkyne to form a triazole.
9. The complex of claim 8 , wherein prior to the cycloaddition reaction, the azide is covalently linked to the valine-citrulline sequence of the cleavable linker that is covalently linked to the 5′ end of the oligonucleotide and the alkyne is provided in a bicyclononyne moiety that further covalently links to the anti-transferrin receptor antibody.
10. The complex of claim 7 , wherein the cleavable linker further comprises one or more polyethylene glycol units.
11. The complex of claim 1 , wherein the anti-transferrin receptor antibody binds to human TfR1 with a K D in a range of 10 −11 M to 10 −8 M.
12. The complex of claim 1 , wherein the anti-transferrin receptor antibody is in the form of a ScFv, Fab fragment, Fab' fragment, F(ab')2 fragment, or Fv fragment.
13. The complex of claim 1 , wherein the anti-transferrin receptor antibody is in the form of a Fab fragment.
14. The complex of claim 1 , wherein the complex promotes transferrin receptor mediated internalization of the oligonucleotide into a muscle cell.
15. The complex of claim 14 , wherein the oligonucleotide brings about reduction in DUX4 expression level in the muscle cell.
16. The complex of claim 14 , wherein the oligonucleotide brings about reduction of expression levels of one or more of ZSCAN1, MBDL3L2 and TRIM43 in the muscle cell.
17. The complex of claim 14 , wherein the muscle cell is a skeletal muscle cell, cardiac muscle cell, or smooth muscle cell.
18. A composition comprising the complex of claim 1 .