IP Library › Granted Patent US 12,590,121
Granted Patent B2
US 12,590,121 · App. 17/401,434 · Granted Mar 31, 2026

Peptide compound with repetitive sequences

Inventors: Charlotte A.E. Hauser (Thuwal, SA); Hepi Hari Susapto (Thuwal, SA)
C07K5/101A61L26/0028A61L26/008A61L27/22A61L27/52A61L31/043A61L31/145C08J3/075C08J3/09C12N5/0068A61K38/00C08J2389/00C12N2513/00C12N2533/50
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Quick Facts
Patent No.
US 12,590,121
App. No.
17/401,434
Granted
Mar 31, 2026
Kind
B2
Abstract

The present disclosure relates to ultrashort peptides capable of forming a gel, to a gel comprising a peptide in accordance with the present disclosure, and to a method of preparing such gel. Such gel is a hydrogel or an organogel. The peptides are suitable bioinks for a bioprinter to build 3D structures through 3D printing as well as other applications.

Claims (244)

1 . An ultrashort peptide consisting of a sequence selected from the group consisting of:

(SEQ ID NO: 2) 

IIFR 

(SEQ ID NO: 3) 

IIFD 

(SEQ ID NO: 4) 

IIFE 

(SEQ ID NO: 5) 

LLFK 

(SEQ ID NO: 6) 

LLFR 

(SEQ ID NO: 7) 

LLFD 

(SEQ ID NO: 8) 

LLFE 

(SEQ ID NO: 9) 

IIZK 

(SEQ ID NO: 10) 

IIZR 

(SEQ ID NO: 11) 

IIZD 

(SEQ ID NO: 12) 

IIZE 

(SEQ ID NO: 13) 

LLZK 

(SEQ ID NO: 14) 

LLZR 

(SEQ ID NO: 15) 

LLZD 

(SEQ ID NO: 16) 

LLZE 

(SEQ ID NO: 17) 

IFFK 

(SEQ ID NO: 18) 

IFFR 

(SEQ ID NO: 19) 

IFFD 

(SEQ ID NO: 20) 

IFFE 

(SEQ ID NO: 21) 

LFFK 

(SEQ ID NO: 22) 

LFFR 

(SEQ ID NO: 23) 

LFFD 

(SEQ ID NO: 24) 

LFFE 

(SEQ ID NO: 25) 

IZZK 

(SEQ ID NO: 26) 

IZZR 

(SEQ ID NO: 27) 

IZZD 

(SEQ ID NO: 28) 

IZZE 

(SEQ ID NO: 29) 

LZZK 

(SEQ ID NO: 30) 

LZZR 

(SEQ ID NO: 31) 

LZZD 

(SEQ ID NO: 32) 

LZZE 

(SEQ ID NO: 33) 

FFIK 

(SEQ ID NO: 34) 

ZZIR 

(SEQ ID NO: 35) 

FFID 

(SEQ ID NO: 36) 

FFIE 

(SEQ ID NO: 37) 

FFLK 

(SEQ ID NO: 38) 

FFLR 

(SEQ ID NO: 39) 

FFLD 

(SEQ ID NO: 40) 

FFLE 

(SEQ ID NO: 41) 

ZZIK 

(SEQ ID NO: 42) 

ZZIR 

(SEQ ID NO: 43) 

ZZID 

(SEQ ID NO: 44) 

ZZIE 

(SEQ ID NO: 45) 

ZZLK 

(SEQ ID NO: 46) 

ZZLR 

(SEQ ID NO: 47) 

ZZLD 

(SEQ ID NO: 48) 

ZZLE 

(SEQ ID NO: 49) 

FIIK 

(SEQ ID NO: 50) 

FIIR 

(SEQ ID NO: 51) 

FIID 

(SEQ ID NO: 52) 

FIIE 

(SEQ ID NO: 53) 

FLLK 

(SEQ ID NO: 55) 

FLLD 

(SEQ ID NO: 56) 

FLLE 

(SEQ ID NO: 57) 

ZIIK 

(SEQ ID NO: 58) 

ZIIR 

(SEQ ID NO: 59) 

ZIID 

(SEQ ID NO: 60) 

ZIIE 

(SEQ ID NO: 61) 

ZLLK 

(SEQ ID NO: 62) 

ZLLR 

(SEQ ID NO: 63) 

ZLLD 

(SEQ ID NO: 64) 

ZLLE 

(SEQ ID NO: 66)

ZZZK,

wherein I is isoleucine, L is leucine, F is phenylalanine, K is lysine, R is arginine, D is aspartic acid, E is glutamic acid, Z is cyclohexylalanine,

wherein each of the sequences is connected to an acetylated or non-acetylated N-terminal protecting group, and may be amidated or non-amidated by a C-terminal protecting group,

wherein the ultrashort peptide is capable of forming a gel by self-assembly,

wherein the N-terminal protecting group is a peptidomimetic molecule,

wherein the N-terminus of the peptidomimetic molecule may be modified with a functional group selected from the group consisting of: carboxylic acid, amide, alcohol, aldehyde, amine, imine, nitrile, an urea analog, phosphate, carbonate, sulfate, nitrate, maleimide, vinyl sulfone, azide, alkyne, alkene, carbohydrate, imide, peroxide, ester, aryl, ketone, sulphite, nitrite, phosphonate, and silane.

2 . The ultrashort peptide recited in claim 1 , wherein the amino acids in the peptide are either L-amino acids or D-amino acids.

3 . The ultrashort peptide recited in claim 1 , wherein the ultrashort peptide is capable of self-assembling into a hydrogel.

4 . The ultrashort peptide recited in claim 1 , wherein the ultrashort peptide is capable of self-assembling into an organogel.

5 . The ultrashort peptide recited in claim 1 , wherein the C-terminal protecting group is selected from the group consisting of: of small molecules, functional groups and linkers.

6 . The ultrashort peptide recited in claim 1 , wherein the C-terminal protecting group is selected from the group consisting of:

polar or non-polar functional groups;

—COOH, —COOR, —COR, —CONHR or —CONRR′ with R and R′ being selected from the group consisting of H, unsubstituted or substituted alkyls, and unsubstituted or substituted aryls;

—NH 2 , —OH, —SH, —CHO, maleimide, imidoester, carbodiimide ester, isocyanate;

small molecules,

comprising sugars, alcohols, hydroxy acids, amino acids, vitamins, or biotin;

linkers terminating in a polar functional group,

comprising ethylenediamine, PEG, carbodiimide ester, or imidoester; and

linkers coupled to small molecules or vitamins,

comprising biotin, sugars, or hydroxy acids.

7 . The ultrashort peptide recited in claim 1 , being stable in aqueous solution at physiological conditions at ambient temperature for a period of time in the range from 1 day to at least 12 months.

8 . A hydrogel comprising the ultrashort peptide recited in claim 1 .

9 . The hydrogel of claim 8 , wherein the hydrogel is stable in aqueous solution at ambient temperature for a period of at least 1 month.

10 . The hydrogel of claim 8 , wherein the hydrogel is characterized by a loss factor tan δ (G″/G′) in the range of 0.08 to 0.17.

11 . The hydrogel of claim 8 , wherein the hydrogel is characterized by a storage modulus G′ from 1250 Pa to 300,000 Pa.

12 . The hydrogel of claim 8 , wherein the hydrogel has a higher mechanical strength than collagen or gelatin.

13 . The hydrogel of claim 8 , wherein the hydrogel is characterized by viscosity in the range of 0.4-0.6 Pa·s.

14 . The hydrogel of claim 8 , comprising fibers of the peptide of claim 1 , the fibers defining a network that is capable of entrapping at least one of a microorganism, a virus particle, a peptide, a peptoid, a protein, a nucleic acid, an oligosaccharide, a polysaccharide, a vitamin, an inorganic molecule, a synthetic polymer, a micro- or nanoparticle, a small organic molecule or a pharmaceutically active compound.

15 . The hydrogel of claim 8 , wherein the hydrogel comprises at least one of a microorganism, a virus particle, a peptide, a peptoid, a protein, a nucleic acid, an oligosaccharide, a polysaccharide, a vitamin, an inorganic molecule, a synthetic polymer, a small organic molecule, a micro- or nanoparticle, or a pharmaceutically active compound entrapped by a network of fibers.

16 . The hydrogel of claim 14 , wherein the fibers are coupled to the at least one of a microorganism, a virus particle, a peptide, a peptoid, a protein, a nucleic acid, an oligosaccharide, a polysaccharide, a vitamin, an inorganic molecule, a synthetic polymer, a small organic molecule, a micro- or nanoparticle, or a pharmaceutically active compound entrapped by the network of fibers.

17 . The hydrogel of claim 8 , wherein the hydrogel is comprised in at least one of a fuel cell, a solar cell, an electronic cell, a biosensing device, a medical device, an implant, a pharmaceutical composition and a cosmetic composition.

18 . The hydrogel of claim 8 , which is injectable.

19 . An organogel comprising the ultrashort peptide recited in claim 1 .

20 . The organogel of claim 19 , wherein the hydrogel is stable in aqueous solution at ambient temperature for a period of at least 1 month.

21 . The organogel of claim 19 , wherein the hydrogel is characterized by a storage modulus G′ from 1250 Pa to 300,000 Pa.

22 . The organogel of claim 19 , wherein the hydrogel has a higher mechanical strength than collagen or gelatin.

23 . The organogel of claim 19 , wherein the hydrogel is characterized by viscosity in the range of 0.4-0.6 Pa·s.

24 . The organogel of claim 19 , comprising fibers of the peptide of claim 1 , the fibers defining a network that is capable of entrapping at least one of a microorganism, a virus particle, a peptide, a peptoid, a protein, a nucleic acid, an oligosaccharide, a polysaccharide, a vitamin, an inorganic molecule, a synthetic polymer, a micro- or nanoparticle, a small organic molecule or a pharmaceutically active compound.

25 . The organogel of claim 19 , wherein the hydrogel comprises at least one of a microorganism, a virus particle, a peptide, a peptoid, a protein, a nucleic acid, an oligosaccharide, a polysaccharide, a vitamin, an inorganic molecule, a synthetic polymer, a small organic molecule, a micro- or nanoparticle, or a pharmaceutically active compound entrapped by a network of fibers.

26 . The organogel of claim 24 , wherein the fibers are coupled to the at least one of a microorganism, a virus particle, a peptide, a peptoid, a protein, a nucleic acid, an oligosaccharide, a polysaccharide, a vitamin, an inorganic molecule, a synthetic polymer, a small organic molecule, a micro- or nanoparticle, or a pharmaceutically active compound entrapped by the network of fibers.

27 . The organogel of claim 19 , wherein the hydrogel is comprised in at least one of a fuel cell, a solar cell, an electronic cell, a biosensing device, a medical device, an implant, a pharmaceutical composition and a cosmetic composition.

28 . The organogel of claim 19 , which is injectable.

29 . A method of preparing a hydrogel or organogel, the method comprising:

dissolving an ultrashort peptide recited in claim 1 in an aqueous solution or an organic solution, respectively.

30 . The method of claim 29 , wherein the dissolved peptide in aqueous or organic solution is further exposed to temperature, wherein the temperature is in the range from 20° C. to 90° C.

31 . The method of claim 29 , wherein the ultrashort peptide is dissolved at a concentration from about 0.01 μg/ml to 100 mg/ml.

32 . The method of claim 29 , wherein the ultrashort peptide is dissolved at a concentration from about 1 mg/ml to 50 mg/ml.

33 . The method of claim 29 , wherein the ultrashort peptide is dissolved at a concentration from about 1 mg/ml to about 20 mg/ml.

34 . A wound dressing or wound healing agent comprising a hydrogel of claim 8 .

35 . A wound dressing or wound healing agent comprising an organogel of claim 19 .

36 . A surgical implant, or stent, the surgical implant or stent comprising a peptide scaffold, wherein the peptide scaffold is formed by a hydrogel of claim 8 .

37 . A surgical implant, or stent, the surgical implant or stent comprising a peptide scaffold, wherein the peptide scaffold is formed by an organogel of claim 19 .

38 . A pharmaceutical composition comprising the ultrashort peptide of claim 1 .

39 . The pharmaceutical composition of claim 38 , further comprising a pharmaceutically active compound.

40 . The pharmaceutical composition of claim 38 , wherein the pharmaceutical composition is provided in the form of a topical gel or cream, a spray, a powder, or a sheet, patch or membrane.

41 . The pharmaceutical composition of claim 38 , wherein the pharmaceutical composition is provided in the form of an injectable solution, a topical gel or cream, a spray, a powder, or a sheet, patch or membrane.

42 . A cosmetic composition comprising the ultrashort peptide of claim 1 .

43 . A biomedical device comprising the ultrashort peptide of claim 1 .

44 . An electronic device comprising the ultrashort peptide of claim 1 .

45 . A kit of parts, the kit comprising a first container with an ultrashort peptide of claim 1 and a second container with an aqueous or organic solution.

46 . The kit of parts of claim 45 , wherein the first container further comprises a pharmaceutically active compound.

47 . The kit of parts of claim 45 , wherein the second container further comprises a pharmaceutically active compound.

48 . The kit of parts of claim 45 , wherein the first container further comprises a pharmaceutically active compound and wherein the second container further comprises a pharmaceutically active compound.

49 . An in vitro or in vivo method of tissue regeneration comprising the steps:

(a) providing a hydrogel of claim 8 ,

(b) exposing the hydrogel to cells which are to form regenerated tissue,

(c) allowing the cells to grow on the hydrogel.

50 . The method of claim 49 , which is performed in vivo, wherein, in step a), the hydrogel is provided at a place in a body where tissue regeneration is intended,

wherein the step a) is performed by injecting the hydrogel at a place in the body where tissue regeneration is intended.

51 . An in vitro or in vivo method of tissue regeneration comprising the steps:

(a) providing an organogel of claim 19 ,

(b) exposing the organogel to cells which are to form regenerated tissue,

(c) allowing the cells to grow on the organogel.

52 . The method of claim 51 , which is performed in vivo, wherein, in step a), the organogel is provided at a place in a body where tissue regeneration is intended,

wherein the step a) is performed by injecting the organogel at a place in the body where tissue regeneration is intended.

53 . A 2D or 3D cell culture substrate comprising a hydrogel of claim 8 .

54 . A 2D or 3D cell culture substrate comprising an organogel of claim 19 .

55 . An ultrashort peptide sequences containing repetitive sequences, the peptide having a general formula selected from:

A n B m X and XB m A n

wherein the total number of amino acids of the ultrashort peptide does not exceed 7 amino acids;

wherein A is an aliphatic amino acids, selected from the group consisting of: isoleucine, leucine or any combination thereof, with n being an integer being selected from 0-5;

wherein B is comprised of at least one aromatic amino acid selected from the group consisting of: tyrosine, tryptophan, phenylalanine, hydrophobic amino acid phenylalanine, or comprised of a peptidomimetic amino acid that is the aliphatic counterpart of the aromatic amino acid;

wherein X is comprised of a polar amino acid, selected from the group consisting of: aspartic acid, glutamic acid, lysine, arginine, histidine, cysteine, serine, threonine, asparagine, and glutamine.

56 . The ultrashort peptide recited in claim 55 , wherein the peptide consists of a sequence selected from the group consisting of:

(SEQ ID NO: 17) 

IFFK 

(SEQ ID NO: 18) 

IFFR 

(SEQ ID NO: 19) 

IFFD 

(SEQ ID NO: 20) 

IFFE 

(SEQ ID NO: 21) 

LFFK 

(SEQ ID NO: 22) 

LFFR 

(SEQ ID NO: 23) 

LFFD 

(SEQ ID NO: 24) 

LFFE 

(SEQ ID NO: 25) 

IZZK 

(SEQ ID NO: 26) 

IZZR 

(SEQ ID NO: 27) 

IZZD 

(SEQ ID NO: 28) 

IZZE 

(SEQ ID NO: 29) 

LZZK 

(SEQ ID NO: 30) 

LZZR 

(SEQ ID NO: 31) 

LZZD 

(SEQ ID NO: 32) 

LZZE 

wherein I is isoleucine, L is leucine, F is phenylalanine, K is lysine, R is arginine, D is aspartic acid, E is glutamic acid, Z is cyclohexylalanine,

wherein each of the sequences may be optionally connected to an acetylated or non-acetylated N-terminal protecting group, and may be amidated or non-amidated by a C-terminal protecting group.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 26, 2021
From: HAUSER, CHARLOTTE A.E.; SUSAPTO, HEPI HARI
To: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
Reel/Frame 057293/0964 →
Continuity (2)
Provisional Application 63067913 · Aug 20, 2020
Related Publication 20220056074A1 · Feb 24, 2022
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