Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
Methods for laser-assisted repositioning of a micro-object and for culturing an attachment-dependent biological cell within a microfluidic device are described herein. Laser illumination is used to controllably create a bubble which repositions the micro-object. Further, methods of culturing an attachment-dependent biological cell are described, where the methods may include laser-assisted repositioning.
1 . A method of repositioning a micro-object disposed in a chamber of a microfluidic device, comprising:
directing laser illumination upon a starting position on an inner surface of the chamber to generate an illumination area, wherein:
the microfluidic device comprises the chamber and a flow region containing a first liquid medium, wherein the chamber comprises an opening to the flow region, and
the starting position is disposed within a sub-region of the chamber distal to the opening to the flow region; and
moving the laser illumination relative to the microfluidic device, thereby changing the position of the illumination area such that, upon completion of the change, the laser illumination is at a final position, wherein the change in the position of the illumination area induces the repositioning of the micro-object at least towards the opening of the chamber;
wherein moving the laser illumination relative to the microfluidic device controllably generates a bubble having a trajectory within the chamber, and wherein the generated bubble induces the repositioning of the micro-object.
2 . The method of claim 1 , wherein the repositioning of the micro-object results in the micro-object being exported from the chamber into the flow region.
3 . The method of claim 2 , wherein the method further comprises exporting the micro-object from the flow region and out of the microfluidic device.
4 . The method of claim 3 , wherein the micro-object is a biological cell, wherein the biological cell is exported out of the microfluidic device and into a culturing vessel, and wherein the biological cell has a probability of viability of at least 60% upon further culturing.
5 . The method of claim 4 , wherein the biological cell has a probability of viability of at least 80% upon further culturing.
6 . The method of claim 1 , wherein the change in the position of the illumination area occurs at a fixed rate, creating a path of laser illumination between the starting position and the final position.
7 . The method of claim 1 , wherein the chamber comprises a sequestration pen comprising:
an isolation region for containing a second fluidic medium, the isolation region having a single opening; and
a connection region fluidically connecting the isolation region to the flow region, wherein the isolation region of the microfluidic sequestration pen is an unswept region of the micro-fluidic device.
8 . The method of claim 7 , wherein the micro-object is disposed within the isolation region of the sequestration pen.
9 . The method of claim 1 , wherein directing the laser illumination further comprises introducing an export medium into the flow region of the microfluidic device, and allowing components of the export medium to diffuse into the chamber.
10 . The method of claim 9 , wherein a temperature of the export medium is about 5° C. to about 10° C. cooler than a temperature of the microfluidic device or a temperature of the first liquid medium.
11 . The method of claim 1 , further comprising using dielectrophoretic (DEP) forces to reposition the micro-object.
12 . The method of claim 1 , wherein changing the position of the illumination area upon an inner surface of the chamber comprises traversing about 20% to about 70% of a length of the inner surface of the chamber.
13 . The method of claim 1 , wherein directing the laser illumination upon the starting position on the inner surface of the chamber comprises illuminating with a laser having a power of less than about 1300 mW.
14 . The method of claim 1 , wherein directing the laser illumination upon the starting position on the inner surface of the chamber comprises illuminating with a laser having a power from about 300 mW to about 600 mW.
15 . The method of claim 1 , wherein the laser illumination is performed for about 40 msec to about 200 msec.
16 . The method of claim 1 , wherein the micro-object is a bead, a biological cell, or a daughter cell derived therefrom.
17 . The method of claim 16 , wherein when the micro-object is a bead, the bead further comprises nucleic acid moieties covalently or non-covalently associated with the bead.
18 . The method of claim 1 , wherein the micro-object is an attachment-dependent cell.
19 . The method of claim 18 , wherein the inner surface of the chamber comprises a surface coating promoting adhesion of the attachment dependent cell.
20 . The method of claim 1 , wherein the inner surface of the chamber comprises a surface coating comprising a mixture of surface modifying ligands; and further wherein at least one of the surface modifying ligands comprises a peptide having an adhesive binding motif.
21 . The method of claim 20 , wherein at least one of the surface modifying ligands does not promote adhesion.
22 . The method of claim 1 , further comprising contacting the micro-object in the chamber with a disengagement reagent prior to directing laser illumination upon the starting position on the inner surface of the chamber.
23 . The method of claim 1 , wherein the chamber includes a plurality of micro-objects disposed therein, and wherein the method further comprises counting the number of micro-objects in the plurality.
24 . The method of claim 1 , wherein the microfluidic device comprises a plurality of chambers and each of the plurality of chambers comprises at least one micro-object disposed therein, further comprising exporting the micro-object(s) in the subset of the plurality of chambers from their respective chambers into the flow region.
25 . The method of claim 1 , wherein directing the laser illumination upon the starting position on the inner surface of the chamber comprises illuminating with a laser having a wavelength of about 720 nm to 1060 nm.