System and method for sorting particles
A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.
1. A multi-channel system for classifying sperm cells according to one or more characteristics of the sperm cells, said system comprising:
a plurality of flow cytometry units each of which is operable to provide sex sorted sperm from a mixture of sperm cells by interrogating a stream of fluid containing said sperm cells using a beam of electromagnetic radiation and detecting a response with at least one sensor operable to generate a output signal indicative of at least one characteristic of the sperm cells;
said units sharing an integrated platform comprising a common processor for detecting digital waveform pulses representing fluorescence emission produced by sperm in response to interrogation by the beam of electromagnetic radiation from each output signal generated from each at least one sensor, extracting features in the detected digital waveform pulses in the form of values representing characteristics of the digital waveform pulses, discriminating the detected digital waveform pulses as a function of their extracted features to determine one or more characteristics of the sperm cells, and classifying sperm cells according to the one or more characteristics
wherein said integrated platform further comprises a common input for controlling operation of the flow cytometry units by the common processor.
2. The system of claim 1 further comprising a common source of electromagnetic radiation comprising a plurality of laser pulses, each pulse having a peak power that is greater than an average power output of a laser and a wavelength in a range of about 350-370 nm.
3. The system of claim 2 wherein said laser emits pulses having a width of about 1-100 picoseconds at a pulse frequency of about 50-150 MHz at a power of about 50-500 milliwatts.
4. The system of claim 2 wherein said common source of said pulsed electromagnetic radiation comprises a single pulsed laser beam.
5. The system of claim 4 further comprising a beam splitting system for splitting the single pulsed laser beam into multiple beams and directing the multiple beams into optics systems of respective flow cytometry units.
6. The system of claim 1 wherein said integrated platform further comprises a common housing, said flow cytometry units comprising interchangeable modules removably mounted in the housing.
7. The system of claim 1 wherein said processor is operable to output an indication of a fluorescence intensity measured by each unit.
8. The system of claim 1 wherein said processor is operable to output an indication of a decision boundary used by each unit for discriminating between sperm cells.
9. The system of claim 1 wherein said flow cytometry units operate in parallel.
10. The system of claim 1 wherein said plurality of flow cytometry units comprises a jet-in-air droplet sorting flow cytometry unit.
11. The system of claim 1 wherein the common processor receives and processes information to permit evaluation of the operation of one unit relative to another unit.
12. The system of claim 1 wherein the processor is operable to process information in real time.