IP Library › Granted Patent US 12,286,638
Granted Patent B2
US 12,286,638 · App. 17/421,678 · Granted Apr 29, 2025

Transgenic barb

Inventors: Alan Blake (Austin, TX); Richard Crockett (Wilton, CT); Aidas Nasevicius (Tampa, FL)
Assignee: GloFish, LLC
C12N15/8509A01K67/0275A01K2217/00A01K2227/40A01K2267/01
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Quick Facts
Patent No.
US 12,286,638
App. No.
17/421,678
Granted
Apr 29, 2025
Kind
B2
Abstract

The present invention relates to transgenic ornamental barbs, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic barbs and methods of providing them to the ornamental fish industry for the purpose of marketing.

Claims (24)

1. A transgenic Barb comprising a chromosomally integrated expression cassette encoding an orange fluorescent protein, wherein the Barb comprises an “Orange Barb 1 transformation event,” sperm comprising the Orange Barb 1 transformation event having been deposited at the ATCC as deposit no. PTA-126571.

2. The transgenic Barb of claim 1 , further defined as a fertile transgenic Barb.

3. The transgenic Barb of claim 1 , wherein the transgenic barb is homozygous for the integrated expression cassette.

4. The transgenic Barb of claim 1 , wherein the transgenic barb is heterozygous for the integrated expression cassette.

5. A method of providing a transgenic Barb to the ornamental fish market, comprising obtaining the transgenic Barb of claim 1 , and distributing the fish to the ornamental fish market.

6. The method of claim 5 , wherein the transgenic barb is distributed by a grower to a commercial distributor.

7. The method of claim 5 , wherein the transgenic barb is distributed by a grower or a commercial distributor to a retailer.

8. The method of claim 7 , wherein the retailer is a multi-product retailer having an ornamental fish department.

9. A method of producing a transgenic Barb comprising:

(a) obtaining a Barb that comprises a chromosomally integrated expression cassette encoding an orange fluorescent protein, wherein the Barb comprises an “Orange Barb 1 transformation event,” sperm comprising the Orange Barb 1 transformation event having been deposited at the ATCC as deposit no. PTA-126571; and

(b) breeding the obtained Barb with a second Barb to provide a transgenic Barb comprising the Orange Barb 1 transformation event.

10. The method of claim 9 , wherein the second Barb is a non-transgenic Barb.

11. A progeny of the transgenic Barb of claim 1 that composes a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the Barb and progeny exhibit fluorescence and comprise a “Orange Barb 1 transformation event,” sperm comprising the Orange Barb 1 transformation event having been deposited at the ATCC as deposit no. PTA-126571.

12. The progeny fish of claim 11 , further defined as a fertile transgenic Barb.

13. The progeny fish of claim 11 , wherein the fish is homozygous for the integrated expression cassette.

14. The progeny fish of claim 11 , wherein the fish is heterozygous for the integrated expression cassette.

15. A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish of claim 11 , and distributing the fish to the ornamental fish market.

16. The method of claim 15 , wherein the progeny fish is distributed by a grower to a commercial distributor.

17. The method of claim 16 , wherein the progeny fish is distributed by a grower or a commercial distributor to a retailer.

18. The method of claim 17 , wherein the retailer is a multi-product retailer having an ornamental fish department.

19. A method of producing a transgenic fish comprising:

(a) obtaining the progeny fish of claim 11 ; and

(b) breeding the obtained progeny fish with a second fish to provide a progeny transgenic Barb comprising the Orange Barb 1 transformation event.

20. The method of claim 19 , wherein the second fish is a non-transgenic fish.

Assignments (2)
SECURITY INTEREST Recorded Apr 23, 2024
From: SPECTRUM BRANDS, INC.; GLOFISH, LLC; SPECTRUM BRANDS PET GROUP, INC.; SPECTRUM BRANDS PET LLC; EMPOWER BRANDS, LLC; UNITED INDUSTRIES CORPORATION
To: ROYAL BANK OF CANADA
Reel/Frame 067201/0653 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 12, 2021
From: BLAKE, ALAN; CROCKETT, RICHARD; NASEVICIUS, AIDAS
To: GLOFISH, LLC
Reel/Frame 057160/0224 →
Continuity (3)
Provisional Application 62790687 · Jan 10, 2019
Provisional Application 62790683 · Jan 10, 2019
Related Publication 20220090126A1 · Mar 24, 2022
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