IP Library › Granted Patent US 12,325,883
Granted Patent B2
US 12,325,883 · App. 17/422,553 · Granted Jun 10, 2025

Loop-mediated isothermal amplification primers for Shiga toxin-producing

Inventors: Tonya D. Bonilla (Woodbury, MN); Neil Percy (St. Paul, MN); Christina A. Barnes (North Hudson, WI)
Assignee: Neogen Food Safety US HoldCo Corporation
C12Q1/689C12Q1/6844C12Q2600/16
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Quick Facts
Patent No.
US 12,325,883
App. No.
17/422,553
Granted
Jun 10, 2025
Kind
B2
Abstract

Primers and methods of amplifying DNA from shiga toxins stx1 and stx2 designed for use in a multiplex method and useful in detecting shiga-toxin producing E. Coli (STEC). The primers being especially designed for use in Loop-mediated Isothermal Amplification (LAMP).

Claims (14)

1. A primer set comprising a primer having at least 80% homology to SEQ ID NO: 3; or

a primer having at least 80% homology to SEQ ID NO: 6, or a combination of the foregoing primers.

2. A primer set of claim 1 , comprising a primer having at least 80% homology to SEQ ID NO: 3 and primer having at least 80% homology to SEQ ID NO: 1.

3. The primer set of claim 1 , further comprising a primer having at least 80% homology to SEQ ID NO. 2.

4. The primer set of claim 1 , further comprising a primer having at least 80% homology to SEQ ID NO. 4.

5. The primer set of claim 1 , further comprising a primer having at least 80% homology to SEQ ID NO: 5.

6. The primer set of claim 1 , further comprising a primer at least 80% homology to SEQ ID NO: 3; and

a primer at least 80% homology to SEQ ID NO: 6.

7. A lyophilized pellet or powder comprising a primer set of claim 1 .

8. A method of amplifying target DNA comprising exposing the target DNA to a primer set of claim 1 under conditions that are suitable for amplification of the target DNA.

9. A primer set of claim 1 , further comprising a primer having at least an 80% homology to SEQ ID 9 or a primer having at least an 80% homology to SEQ ID 12, or both.

10. A primer set of claim 9 , further comprising a primer having at least an 80% homology to SEQ ID 7, a primer having at least an 80% homology to SEQ ID 10, a primer having at least an 80% homology to SEQ ID 8, a primer having at least 80% homology to SEQ ID 11, or more than one of the foregoing.

11. A primer set of claim 9 , comprising a primer having at least 80% homology to SEQ ID 9 and a primer having at least 80% homology to SEQ ID 12.

12. A primer set of claim 11 , further comprising a primer having at least 80% homology to SEQ ID 7, a primer having at least 80% homology to SEQ ID 10, or both.

Assignments (4)
SECURITY INTEREST Recorded Sep 2, 2022
From: NEOGEN CORPORATION; NEOGEN FOOD SAFETY US HOLDCO CORPORATION
To: JPMORGAN CHASE BANK, N.A., AS COLLATERAL AGENT
Reel/Frame 061372/0264 →
PATENT ASSIGNMENT AGREEMENT Recorded Sep 1, 2022
From: 3M INNOVATIVE PROPERTIES COMPANY
To: GARDEN US HOLDCO CORPORATION
Reel/Frame 061365/0688 →
CHANGE OF NAME Recorded Sep 1, 2022
From: GARDEN US HOLDCO CORPORATION
To: NEOGEN FOOD SAFETY US HOLDCO CORPORATION
Reel/Frame 061370/0437 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 13, 2021
From: BONILLA, TONYA D.; PERCY, NEIL; BARNES, CHRISTINA A.
To: 3M INNOVATIVE PROPERTIES COMPANY
Reel/Frame 056837/0412 →
Continuity (3)
Provisional Application 62847456 · May 14, 2019
Provisional Application 62792670 · Jan 15, 2019
Related Publication 20230183819A1 · Jun 15, 2023
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