Anti-elastin antibodies and methods of use
Antibodies and antigen binding fragments thereof that specifically recognize and bind an epitope of elastin that is exposed and accessible in degraded elastic fiber are described. The antibodies and/or antigen binding fragments can be operably linked to a secondary component, including biologically active agents such as therapeutics and/or imaging agents. Optionally, the antibodies and/or antigen binding fragments thereof can be attached to a surface of a carrier, such as a particle, for specific binding and delivery of the carried agents to degraded elastic fiber.
1. An antibody or antigen binding fragment thereof which binds to degraded elastin, the antibody or antigen binding fragment thereof comprising the amino acid sequence of SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 27, SEQ ID NO: 29, and SEQ ID NO: 31.
2. The antibody or antigen binding fragment thereof of claim 1 , wherein the antibody is a monoclonal antibody.
3. The antibody or antigen binding fragment thereof of claim 1 , wherein the antibody is a polyclonal antibody.
4. The antibody or antigen binding fragment thereof of claim 1 , wherein the antibody or antigen binding fragment thereof is an antibody that is a fully human antibody, a non-human antibody, a chimeric antibody, a humanized antibody, or a single domain antibody.
5. The antibody or antigen binding fragment thereof of claim 1 , wherein the antibody or antigen binding fragment thereof is an antigen binding fragment that comprises a Fab, a Fab′, a F(ab′)2, a Fv, a disulfide linked Fv, or a scFv.
6. A composition comprising the antibody or antigen binding fragment thereof of claim 1 directly or indirectly operably linked to a secondary material.
7. The composition of claim 6 , wherein the secondary material comprises a biologically active agent.
8. The composition of claim 7 , wherein the biologically active agent comprises an anticoagulant, an antiplatelet, an anti-inflammatory, an SMC proliferation inhibitor, a matrix metalloproteinase inhibitor, a cathepsin inhibitor, a cytostatic agent, an anti-oxidant, a collagen stabilizing agent, an elastin-stabilizing agent, an elastin regeneration agent, a cytokine, an enzyme, a chemokine, a radioisotope, a toxin, an immunomodulatory agent, a chelator, a nucleic acid construct, a chemotherapeutic agent, an elastin crosslinking agent or a tropoelastin crosslinking agent.
9. The composition of claim 6 , wherein the secondary material comprises an imaging agent.
10. The composition of claim 9 , wherein the imaging agent comprises a photoactivatable agent, a fluorophore, a radioisotope, a bioluminescent peptide, a fluorescent tag, a fluorescent peptide, an affinity label, an enzymatic label, an MRI agent, a gold particle, an x-ray opaque substance, or an isotopic label.
11. The composition of claim 6 , wherein the secondary material comprises a carrier.
12. The composition of claim 11 , wherein the carrier comprises a liposome.
13. The composition of claim 11 , wherein the carrier is a particle.
14. The composition of claim 13 , wherein the particle is a nanoparticle.
15. The composition of claim 13 , wherein the particle comprises a degradable polymer.
16. The antibody or antigen binding fragment thereof of claim 1 , comprising the amino acid sequence of SEQ ID NO: 7.
17. The antibody or antigen binding fragment thereof of claim 1 , comprising the amino acid sequence of SEQ ID NO: 25.
18. A method of binding to degraded elastin fiber comprising contacting a degraded elastic fiber with an antibody or antigen binding fragment thereof of claim 1 , wherein the antibody or antigen binding fragment thereof is operably linked to a secondary material.
19. The method of claim 18 , wherein the secondary material comprises a carrier and a biologically active agent incorporated into the carrier, wherein the biologically active agent is released from the carrier following the binding of the antibody or antigen binding fragment thereof to the degraded elastic fiber.
20. The method of claim 18 , the secondary material comprising an imaging agent.